NUTRITIONAL REGUILATION OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-2 GENE EXPRESSION IN THE CHICKEN

鸡胰岛素样生长因子结合蛋白2基因表达的营养调控

• 批准号: 11660282
• 负责人: KITA Kazumi
• 金额: $ 2.3万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660282/
• 关键词: IGFBP-2; Gizzard; Brain; Dietary protein; Amino acid; Gene expression; Insulin; Chickens; 大豆抽出蛋白質; カゼイン; インスリン様成長因子; インスリン様成長因子結合蛋白質

The influence of feeding with experimental diets containing different dietary protein quantity and quality on changes in IGFBP-2 gene expression in various tissues of young chickens was examined. The hepatic IGFBP-2 mRNA was not detected among all treatments. When chickens fed a 5% soybean diet, brain IGFBP-2 mRNA level was lower than that of chickens fed a 20% soybean diets supplemented with essential amino acids. In the gizzard, IGFBP-2 mRNA in the group of a 20% soybean diets supplemented with essential amino acids was significantly lower than other groups. There was no significant difference in IGFBP-2 gene expression in the kidney among all treatments. These results suggest that the difference in dietary protein quantity affect IGFBP-2 gene expression in brain and gizzard, and that the difference in dietary protein quality affects IGFBP-2 gene expression in only gizzard. The expression of IGFBP-2 gene might be less responsive to the difference in dietary protein quality than that in dietary protein quantity.The changes in tissue IGFBP-2 gene expression of young fasted chickens followed by refeeding a commercial diet or by administrating bovine insulin was examined. Hepatic IGFBP-2 mRNA was significantly increased by fasting for 2 days and decreased by refeeding for 6 h. Insulin administration also decreased hepatic IGFBP-2 gene expression stimulated by fasting to the level of refed chickens. IGFBP-2 mRNA in the gizzard was significantly increased by fasting for 2 days and tended to decrease by refeeding for 6 h. Insulin administration decreased gizzard IGFBP-2 gene expression lower than the level of refed chickens. There was no significant difference in IGFBP-2 mRNA in the brain and kidney among all treatments. These results suggest that the changes in IGFBP-2 gene expression in the liver and gizzard by fasting and refeeding might be partly regulated by the alteration in plasma insulin concentration.

研究了含有不同饮食蛋白量和质量的实验饮食对幼鸡各种组织中IGFBP-2基因表达变化的影响的影响。在所有治疗中均未检测到肝IGFBP-2 mRNA。当喂食5％大豆饮食的鸡时，脑IGFBP-2 mRNA水平低于喂食20％大豆饮食的鸡的鸡肉水平。在Gizzard中，补充了必需氨基酸的20％大豆饮食组中的IGFBP-2 mRNA明显低于其他组。在所有治疗中，肾脏中的IGFBP-2基因表达没有显着差异。这些结果表明，饮食蛋白质量的差异会影响脑和Gizzard中IGFBP-2基因的表达，而饮食蛋白质质量的差异仅影响Gizzard中的IGFBP-2基因表达。 IGFBP-2基因的表达对饮食蛋白质质量的差异可能比饮食蛋白量的差异更少。肝IGFBP-2 mRNA通过禁食2天显着增加，并通过重新添加6小时减少。胰岛素给药还降低了肝IGFBP-2基因表达，该基因表达通过禁食到ref鸡的水平。 gizz剂中的IGFBP-2 mRNA通过禁食2天显着增加，并通过重新添加6小时降低。胰岛素给药降低了igfbp-2基因表达低于ref鸡的水平。在所有治疗中，脑和肾脏中的IGFBP-2 mRNA均无显着差异。这些结果表明，禁食和重喂养在肝脏和gizz中的IGFBP-2基因表达的变化可能部分受到血浆胰岛素浓度的改变。