NUTRITIONAL REGUILATION OF INSULIN-LIKE GROWTH FACTOR BINDING PROTEIN-2 GENE EXPRESSION IN THE CHICKEN

鸡胰岛素样生长因子结合蛋白2基因表达的营养调控

• 批准号: 11660282
• 负责人: KITA Kazumi
• 金额: $ 2.3万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11660282/
• 关键词: IGFBP-2; Gizzard; Brain; Dietary protein; Amino acid; Gene expression; Insulin; Chickens; 大豆抽出蛋白質; カゼイン; インスリン様成長因子; インスリン様成長因子結合蛋白質

The influence of feeding with experimental diets containing different dietary protein quantity and quality on changes in IGFBP-2 gene expression in various tissues of young chickens was examined. The hepatic IGFBP-2 mRNA was not detected among all treatments. When chickens fed a 5% soybean diet, brain IGFBP-2 mRNA level was lower than that of chickens fed a 20% soybean diets supplemented with essential amino acids. In the gizzard, IGFBP-2 mRNA in the group of a 20% soybean diets supplemented with essential amino acids was significantly lower than other groups. There was no significant difference in IGFBP-2 gene expression in the kidney among all treatments. These results suggest that the difference in dietary protein quantity affect IGFBP-2 gene expression in brain and gizzard, and that the difference in dietary protein quality affects IGFBP-2 gene expression in only gizzard. The expression of IGFBP-2 gene might be less responsive to the difference in dietary protein quality than that in dietary protein quantity.The changes in tissue IGFBP-2 gene expression of young fasted chickens followed by refeeding a commercial diet or by administrating bovine insulin was examined. Hepatic IGFBP-2 mRNA was significantly increased by fasting for 2 days and decreased by refeeding for 6 h. Insulin administration also decreased hepatic IGFBP-2 gene expression stimulated by fasting to the level of refed chickens. IGFBP-2 mRNA in the gizzard was significantly increased by fasting for 2 days and tended to decrease by refeeding for 6 h. Insulin administration decreased gizzard IGFBP-2 gene expression lower than the level of refed chickens. There was no significant difference in IGFBP-2 mRNA in the brain and kidney among all treatments. These results suggest that the changes in IGFBP-2 gene expression in the liver and gizzard by fasting and refeeding might be partly regulated by the alteration in plasma insulin concentration.

研究了饲喂含有不同蛋白质数量和质量的实验日粮对幼鸡各组织IGFBP-2基因表达变化的影响。所有处理中均未检测到肝脏IGFBP-2 mRNA。当鸡饲喂5%大豆日粮时，其大脑IGFBP-2 mRNA水平低于饲喂20%大豆日粮并补充必需氨基酸的鸡。在砂囊中，补充必需氨基酸的20%大豆日粮组中IGFBP-2 mRNA显着低于其他组。所有治疗组中肾脏中 IGFBP-2 基因表达没有显着差异。这些结果表明，膳食蛋白质数量的差异影响脑和砂囊中IGFBP-2基因的表达，而膳食蛋白质质量的差异仅影响砂囊中IGFBP-2基因的表达。 IGFBP-2基因的表达对日粮蛋白质质量差异的反应可能不如对日粮蛋白质数量差异的反应。禁食幼鸡在重新饲喂商业日粮或施用牛胰岛素后组织IGFBP-2基因表达的变化检查了。禁食2天后肝脏IGFBP-2 mRNA显着增加，再进食6小时后肝脏IGFBP-2 mRNA显着降低。注射胰岛素还可将禁食刺激的肝脏 IGFBP-2 基因表达降低至禁食鸡的水平。禁食2天后砂囊中IGFBP-2 mRNA显着增加，再喂食6小时后趋于减少。施用胰岛素使砂囊 IGFBP-2 基因表达降低，低于进食鸡的水平。所有治疗组中大脑和肾脏中 IGFBP-2 mRNA 没有显着差异。这些结果表明，禁食和再进食引起的肝脏和砂囊中 IGFBP-2 基因表达的变化可能部分受到血浆胰岛素浓度变化的调节。