Development of the nano device for medical use based on the Biochemical IC

基于生化IC的医疗用纳米器件的开发

基本信息

批准号：
15206027
负责人：
IKUTA Koji
金额：
$ 32.2万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We studied the novel conceptual device, "Biochemical IC chip," from the basic material stage to the applicable cell-fusion chip stage under systematic approach. The purposes of this research are diversification and application to biology, which all the distances of the various synthesis and analysis in the biochemistry area could downsize to micro-size domain.Biochemical IC chip for cell-five protein synthesis, originally developed and verified as use for luciferase, a luminous protein of firefly in the year of 2001, was evolved to be synthesized general and well known marker protein Green fluorescence protein (GFP) continuously for along-term period. Through this new evidence, utility and generality of the Biochemical IC chip-set for cull-free protein synthesis was verified. As a basic technology of a mien) device with a built-in cell, we found the condition which the cell could be cultivated in the micro device among the appointed time, by studying several point, hunt up the photocur … More able resin with biocompatibility, surface modification, surface structure, fabrication strategy of the microstructure, and so on.This basic study is essential because photo-initiator, containing in the photocurable polymers, prevent cell growth on the surface of the device. We achieved the processing resolution of less than 10 micrometer, which high-resolution is required to construct the free-surface microstereolithography, essential to fabricate hybrid structure for biochemical IC chip. This new technology is contributed to not only acquire 10-micrometer-diameter micro pipe, but also improvement of the cell adhesion.We proposed and developed "optically driven nano-machine," which is controlled by the laser trapping remotely The nano-machine has movable elements and is fabricated by high-speed two-photon-absorption microstereolithography to realize a cell operation under the microscope in advance. We also verified its availability by fabricating nano-tweezer, nano-needle of 2 D.O.F.,10 micrometer-length nano-manipulator of 3 D.O.F, and so on.We also developed three-dimensional microfabrication system for biodegradable polymers, especially poly (lactic acid), for the purpose of the implantable use as a drug release chip of the biochemical IC. The resolution attained are 50 micrometer, but the fabricated device has good biocompatibility, because we never employ toxic solvents. Less

我们以系统化的方式研究了新型概念装置“生化IC芯片”，从基础材料阶段到适用的细胞融合芯片阶段，本研究的目的是多样化和应用于生物学，这涵盖了各种合成的所有距离。用于五细胞蛋白合成的生化IC芯片，最初于2001年开发并验证用于萤火虫发光蛋白荧光素酶，现已发展到通过这一新证据，可以长期连续合成通用且众所周知的标记蛋白绿色荧光蛋白（GFP），验证了生化IC芯片组作为无剔除蛋白合成的基础技术的实用性和通用性。一种内置细胞的装置，我们发现了细胞可以在指定时间内在微型装置中培养的条件，通过研究几个点，寻找光固化…更具有生物相容性、表面改性、表面活性的树脂结构，微观结构的制造策略，这项基础研究是至关重要的，因为光固化聚合物中含有的光引发剂会阻止细胞在器件表面生长，我们实现了小于10微米的加工分辨率，而这是构建器件所需的高分辨率。自由表面微立体光刻技术，对于制造生化IC芯片的混合结构至关重要，这项新技术不仅有助于获得10微米直径的微管，而且有助于提高细胞粘附性。我们提出并开发了这种技术。通过激光捕获远程控制的“光驱动纳米机器”，该纳米机器具有可移动元件，并通过高速双光子吸收微立体光刻技术制造，实现了显微镜下的细胞操作。通过制作纳米镊子、二自由度纳米针、10微米长三自由度纳米机械手等，我们还开发了纳米镊子、二自由度纳米针、三自由度纳米机械手等。生物可降解聚合物，特别是聚乳酸的三维微制造系统，用于植入式生化IC的药物释放芯片，所达到的分辨率为50微米，但所制造的器件具有良好的生物相容性。切勿使用有毒溶剂。