Development of the nano device for medical use based on the Biochemical IC

基于生化IC的医疗用纳米器件的开发

基本信息

批准号：
15206027
负责人：
IKUTA Koji
金额：
$ 32.2万
依托单位：
Nagoya University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

项目摘要

We studied the novel conceptual device, "Biochemical IC chip," from the basic material stage to the applicable cell-fusion chip stage under systematic approach. The purposes of this research are diversification and application to biology, which all the distances of the various synthesis and analysis in the biochemistry area could downsize to micro-size domain.Biochemical IC chip for cell-five protein synthesis, originally developed and verified as use for luciferase, a luminous protein of firefly in the year of 2001, was evolved to be synthesized general and well known marker protein Green fluorescence protein (GFP) continuously for along-term period. Through this new evidence, utility and generality of the Biochemical IC chip-set for cull-free protein synthesis was verified. As a basic technology of a mien) device with a built-in cell, we found the condition which the cell could be cultivated in the micro device among the appointed time, by studying several point, hunt up the photocur … More able resin with biocompatibility, surface modification, surface structure, fabrication strategy of the microstructure, and so on.This basic study is essential because photo-initiator, containing in the photocurable polymers, prevent cell growth on the surface of the device. We achieved the processing resolution of less than 10 micrometer, which high-resolution is required to construct the free-surface microstereolithography, essential to fabricate hybrid structure for biochemical IC chip. This new technology is contributed to not only acquire 10-micrometer-diameter micro pipe, but also improvement of the cell adhesion.We proposed and developed "optically driven nano-machine," which is controlled by the laser trapping remotely The nano-machine has movable elements and is fabricated by high-speed two-photon-absorption microstereolithography to realize a cell operation under the microscope in advance. We also verified its availability by fabricating nano-tweezer, nano-needle of 2 D.O.F.,10 micrometer-length nano-manipulator of 3 D.O.F, and so on.We also developed three-dimensional microfabrication system for biodegradable polymers, especially poly (lactic acid), for the purpose of the implantable use as a drug release chip of the biochemical IC. The resolution attained are 50 micrometer, but the fabricated device has good biocompatibility, because we never employ toxic solvents. Less

我们研究了新型概念设备“生化IC芯片”，从基本材料阶段到系统方法的适用细胞融合芯片阶段。 The purposes of this research are diversification and application to biology, which all the distances of the various synthesis and analysis in the biochemistry area could downsize to micro-size domain.Biochemical IC chip for cell-five protein synthesis, originally developed and verified as use for luciferase, a luminous protein of Firefly in the year of 2001, was evolved to be synthesized general and well known marker protein Green fluorescence蛋白质（GFP）连续持续期。通过这一新证据，验证了生化IC CHIP键的效用和通用性用于无毛蛋白质合成。作为具有内置电池的MIEN设备的基本技术，我们发现了通过研究几个点，寻找具有生物相容性，表面修饰，表面结构，微观结构的制造策略等的更有能力的树脂，可以在指定时间之间在微型设备中种植细胞的条件。这项基础研究是必不可少的，因为光吸光器中包含可光化聚合物中的光吸收剂可以防止设备表面的细胞生长。我们实现了少于10千米的处理分辨率，这需要高分辨率来构建自由表面的微骨术，这对于制造生化IC芯片的混合结构必不可少。 This new technology is contributed to not only acquire 10-micrometer-diameter micropipe, but also improvement of the cell adhesive.We proposed and developed "optically driven nano-machine," which is controlled by the laser trapping remotely The nano-machine has movable elements and is fabricated by high-speed two-photon-absorption microstereolithography to realize a cell operation under the microscope in advance.我们还通过制造2 d.o.f.的纳米螺旋，纳米针，10微米长的纳米纳米针，3 d.o.f的10微米长纳米 - 纳米操作器，等等。我们还开发了生物降解聚合物的三维微型制造系统，用于boi off premable premape of the Ic Chip chip of the Ic Chip chip chip of the Icchip of the IC的生物降解聚合物。达到的分辨率为50千米，但是制造的设备具有良好的生物相容性，因为我们从不雇用有毒解决方案。较少的