DEVELOPMENT OF BIOCHEMICAL IC CHIPS

生化IC芯片开发

• 批准号: 13305017
• 负责人: IKUTA Koji
• 金额: $ 36.03万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13305017/
• 关键词: Biochemical IC; Micro Stereolithography; Photo Curable Polymer; Biocompatible Materials; Tailor-made Medicine; Implantable Micro Device; Micro Chemical Analysis System; Optical Drive; テーラーメイド製薬

Biochemical IC chips for future micro/nano chemical device have been investigated. We took a systematic research approach for development compared with conventional research project.1) New fabrication process to create real three dimensional micro/nano structures has been developed. The micro/nano stereo lithography process originally developed in Ikuta's laboratory was further advanced for this project. Nano scale needle and tweezers were fabricated and driven by laser trapping. Smooth remote drive and ft Newton scale force sensing were demonstrated experimentally.2) New chip-sets of biochemical IC chips have been successfully developed and their functions were verified. Cell-free protein synthesis with advanced micro pump chips was succeeded. Several proteins have been synthesized from DNA without any living cell. This function is the key technology for not only post genome research but also order-made medicine based.3) Homogenizer chips to destroy cell and tissue in micro scale without damaging cell protein. Simple and effective coupling method between biochemical IC chips was developed. The silicon-rubber coupling can make a tight sealing. These results directly contribute to real total micro analysis process and devices.4) Artificial kidney device utilizing micro stereo lithography has been developed. Miniaturization and rat test were carried out successfully in Prof. Nagakura's lab.5) New technique for cell biology by using micro fluidics has been proposed and verified by Prof. Sokabe and Prof. Naruse. Since many new knowledge in life science were obtained through the new approach, it was made clear that micro chemical devices such as biochemical IC chips can contribute to basic life science as well as biomedical applications.

已经研究了用于未来微型/纳米化学装置的生化IC芯片。与常规研究项目相比，我们采用了一种系统的开发方法。1）开发了创建实际三维微型/纳米结构的新制造过程。该项目最初在Ikuta的实验室中开发的微/纳米立体声光刻过程。纳米秤针和镊子是由激光诱捕制造和驱动的。经过实验证明了光滑的远程驱动器和牛顿量表的力传感。2）生化IC芯片的新芯片已经成功地开发出来，并验证了其功能。无细胞的蛋白质与高级微泵芯片成功。几种蛋白质已从没有任何活细胞的DNA中合成。该功能不仅是基因组研究后，而且是基于订单医学的关键技术。3）均质芯片以微小破坏细胞和组织而不会损害细胞蛋白。开发了生化IC芯片之间的简单有效的耦合方法。硅橡胶耦合可以使密封紧密。这些结果直接有助于实际的总微分析过程和设备。4）已经开发了使用微观立体光刻的人工肾脏装置。在Nagakura教授的实验室成功进行了微型化和大鼠测试。5）通过使用微流体的新技术，已由Sokabe教授和Naruse教授提出并验证。由于通过新方法获得了许多生活科学方面的新知识，因此很明显，诸如生化IC芯片之类的微型化学设备可以为基本的生命科学以及生物医学应用做出贡献。

项目成果

K. Ikuta, K. Ikeda, A. Takahashi, S. Maruo: "Artificial cellular device with cell-free protein synthesis ability constructed by chemical IC chips"Proc. of the IEEE Conference on Nanotechnology (IEEE NANO 2001). 134-139 (2001)

K. Ikuta，K. Ikeda，A. Takahashi，S. Maruo：“由化学IC芯片构建的具有无细胞蛋白质合成能力的人工细胞装置”Proc。

S.Maruo, K.Ikuta, H.Korogi: "Optically driven micromanipulation tools fabricated by two-photon microstereolithography"Mat. Res. Soc. Symp. Proc.. 739(In press). (2003)

S.Maruo、K.Ikuta、H.Korogi：“通过双光子微立体光刻技术制造的光学驱动微操作工具”Mat。

伊丹正浩、長倉俊明 他: "浸透圧バルブのマスプロダクトの検討"医用電子と生体工学. 39. 540 (2001)

Masahiro Itami、Toshiaki Nagakura 等：“渗透阀批量产品的研究”医疗电子和生物工程 39. 540 (2001)。

野上真子、長倉俊明 他: "多源視による定量的3次元画像の再構成の研究"医用電子と生体工学. 39. 540 (2001)

Mako Nogami、Toshiaki Nagakura 等人：“使用多源观察进行定量三维图像重建的研究”《医疗电子与生物工程》39. 540 (2001)。

生田幸士(分担執筆): "マイクロマシン技術総覧"産業技術サービスセンター. 753 (2003)

生田浩二（撰稿人）：《微型机械技术概述》工业技术服务中心753（2003）。