Analysis of the primary structure of summer-morph-producing hormone and photoperiodic and endocrine regulatory mechanism in the butterfly

蝴蝶夏季形态产生激素的一级结构及光周期和内分泌调节机制分析

基本信息

批准号：
14540628
负责人：
ENDO Katsuhiko
金额：
$ 1.98万
依托单位：
Yamaguchi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

a) The small copper butterfly, Lycaena phlaeas daimio, has pupal beige/black polymorphism, development of which is found to be controlled in all apparent association with the development of adult seasonal polymorphism (spring and summer morphs) by photoperiod and temperature given in the larval stages. That is, pupae of beige and black types developed under long-day and short-day conditions tended to develop alto brown-winged and red-winged adults, respectively. In addition, a large proportion of long-day pharate pupae which had been chilled at 4℃ for 5 days developed into pupae whose head-thoracic complexes and abdomens were judged to be black and intermediate types, respectively. They developed into adults with more reddish wings as compared to those developed from the unchilled controlsb) Analysis of the primary structure of Bombyx summer-morph-producing hormone (SMPH) Foamy thousand brain-soboesophageal ganglion complexes were obtaind from the silkmoths, Bombyx mori, by dissection in 0.9% NaCl. A cerebral neuropeptide showing SMPH-activity in the Asian comma butterfly, Polygonia c-aureum. Crude extracts of SMPH-active peptide were purified by a gel-filtration and six steps of reverse-phased HPLC.A peptide fraction showing a suigle peak in absorbance of 220nm was obtained twice and analyzed amino acid sequence of N-terminal of the SMPH-active peptide. As the results, we succeeded to obtain the arrangement of 10 amino acids from the N-terminal of the peptides. However, amino acid sequences of N-terminals obtained by the first and second trials were different partly. We should try to purify the SMPH-active peptide main from Br-SG complexes of the silkmoth and analyze amino acid sequence of the N-terminals

a）小铜蝴蝶，lycaena phlaeas daimio，与photoperiod and teverator幼虫阶段（即米色和黑色类型下的phote toperiod od theperpae themio daimio daimio daimio daimio daimio daimio daimio daimio daimio daimio daimio daimio daimio而且，短期的条件倾向于发展中音棕翅和有翼和GED成年人，此外，在4°C的天数中散发出长时间的Pharate Pupae的大量建议软管软管的软管头膜被认为是黑色的近媒体，它们与来自未chilled的Controlsb开发的翅膀相比，它们具有更多的翅膀）。 Soboespheageal神经节膜，bombyx mori，通过0.9％NaCl中的naCl中的dledigonia c-aureum。战争的两倍并分析了结果的N末端的氨基酸秘密，我们。丝绸的丝绸，分析N末端的氨基酸序列