Analyses of primary structure and production cells of a Bombyx cerebral neuropeptide producing summer morphs in Polygonia c-aureum

金黄色蓼产生夏季形态的家蚕脑神经肽的初级结构和生产细胞分析

• 批准号: 12640662
• 负责人: ENDO Katsuhiko
• 金额: $ 2.3万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12640662/
• 关键词: seasonal morphs of butterfly; summer-morph-producing hormone; purification and isolation; primary structure of hormone; establishment of bioassay mentod; Papilio xuthus; Bombyx mori; brain-suboesophageal ganglion; 精製・単離; 一時構造の解析; 脳・食道下神経節; チョウ目

Molecular weight (MW) of Bombyx cerebral neuroopeptide showing summer-morph-producing hormone (SMPH) activity was estimated to be 4,500 by gel-filtration using a Sephadex G-50 column. By a gel-filtration using a Superose-12 column, MW of the SMPH-active peptide extracted and purified partially in different ways as above was estimated to be 12,000. It may be that SMPH-active peptide forms a dimmer in this condition. We collected 20,000 of brain-suboesophageal ganglion complexes of adult Bombyx mori. But, our experiments did not go on now, because we could not provide Polygonia short-day pupae for assaying SMPH-actovity in winter and spring.Papilio xuthus has seasonal morphs, spring and summer morphs, whose development is determined by photoperiod and temperature. In this project, the summer-morph development is found to be mediated by the SMPH. We succeeded in establishing an assaying method for SMPH using diapausing pupae of P.xuthus. Now, we are going to try our experiments on this bioassay method.

通过使用 Sephadex G-50 柱进行凝胶过滤，显示出夏季形态产生激素 (SMPH) 活性的家蚕脑神经肽的分子量 (MW) 估计为 4,500。通过使用Superose-12柱的凝胶过滤，以上述不同方式部分提取和纯化的SMPH活性肽的MW估计为12,000。 SMPH 活性肽可能在这种情况下形成二聚体。我们收集了 20,000 个成年家蚕的脑-食管下神经节复合体。但是，我们的实验现在没有继续进行，因为我们无法提供Polygonia短日照蛹用于测定冬季和春季的SMPH活性。 Papilio xuthus有季节性形态，春季和夏季形态，其发育由光周期和温度决定。在这个项目中，发现夏季变形的发育是由 SMPH 介导的。我们成功建立了利用 P.xuthus 滞育蛹检测 SMPH 的方法。现在，我们将尝试对这种生物测定方法进行实验。

项目成果

Takamasa Ito: "Evidence for the presence of the summer-morph-producing hormone in the swallowtail butterfly, Papilio xuthus L (Lepidoptera : Papilionidae)"Zoological Science. 18. 1117-1122 (2001)

Takamasa Ito：“燕尾蝴蝶 Papilio xuthus L（鳞翅目：凤蝶科）中存在产生夏季变形激素的证据”动物学。

Yamanaka et al.: "Neuroendocrine regulation of seasonal morph development in bivoltine race (Daizo) of the silkmoth, Bombyx mori L."Journal of Insect Physiology. 46. 803-808 (2000)

Yamanaka 等人：“蚕蛾 Bombyx mori L 的二化种族 (Daizo) 季节性形态发育的神经内分泌调节。”昆虫生理学杂志。

松井茂生 ら: "山口県美祢郡美東町台山(山口県営育成牧場)の蛾類"山口生物. 27(発表予定). (2000)

Shigeo Matsui 等：“山口县美峰郡醍醐町代山的蛾（山口县育种场）”Yamaguchi Bio. 27（待出版）。

Muranaka K, Yamanaka A, Tanaka H, shahjahan R.M, Endo K.: "Effects of juvenile hormone-I and adult brain-suboesophageal ganglion extracts on ovarain maturation of reproductive diapausing females of Polygonia c-aureum"Asian Information. 1(in press). (2002)

Muranaka K、Yamanaka A、Tanaka H、shahjahan R.M、Endo K.：“保幼激素-I 和成体脑食管下神经节提取物对金黄色蓼生殖滞育雌性卵巢成熟的影响”亚洲信息。

Akira Yamanaka: "Effects of hormonal factor on pupal cuticle coloration in the swallowtail butterfly, Papilia xuthus"Proc.Arthropod.Embryol.Soc.Jpn.. 35. 13-16 (2000)

Akira Yamanaka：“激素因素对燕尾蝴蝶 Papilia xuthus 蛹角质层着色的影响”Proc.Arthropod.Embryol.Soc.Jpn.. 35. 13-16 (2000)