Purification and Isolation of Polygonia Summer-Morph-Producing Hormone and Bombyx SMPH-Active Substance.

蓼夏变形激素和家蚕 SMPH 活性物质的纯化和分离。

• 批准号: 01540601
• 负责人: ENDO Katsuhiko
• 金额: $ 1.22万
• 依托单位: Yamaguchi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01540601/
• 关键词: summer morph; hormone; Polygonia c-aureum; Bombyx mori; purification; 光周性; 人工飼料; カイコガ; イオン交換クロマトグラフィ-

Our present research results consists of 4 parts, the first of which is the characterization of the photoperiodic responses of test animal, Polygoniac-aureum Yamaguchi strain. The second and third parts are the improvement of artificial diet for Un seasonal and mass reproduction of P. c-aureum and the determination of the Bombyx stage at which the brains extracting a summer-morph-producing-hormone-active substance. The 4th part is the methodological confirmation for employing ion-exchange chromatography on a course of Bombyx Summer-Morph-Producing-Hormone (SMPH) active substance purification.The first of third parts of this project were mostly established in this project starting on April, 1989. The 4th part, maybe most important part of this project, was also established mostly, we thought, but remains some problems. in it. That is, we could obtain Bombyx moth brains of more than 20 thousands by October, 1990, when P. c-aureum season starting from May and ending at October was mostly finished. So, we remained an important problem for establishing the ion-exchange chromatography as a purification step of the Bombyx SMPH-active substance

我们目前的研究结果由4个部分组成，其中首先是测试动物Polyoniac-Aureum Yamaguchi菌株的光周期反应的表征。第二部分和第三部分是改善人工饮食，用于联合国季节性和大规模繁殖C-caureum，以及确定Bombyx阶段的大脑，在该阶段中提取了夏季刺激性的激素活性物质。第四部分是在Bombyx夏季产生的荷尔蒙（SMPH）活动物质纯化的方法上采用离子交换色谱法的方法学确认。该项目的第三部分主要是在4月开始的该项目中建立的。 1989年。我们认为，第四部分，也许是该项目最重要的部分，也是大部分是建立的，但仍然存在一些问题。在其中。也就是说，到1990年10月，当P. C-Aureum季节从5月开始，到10月的结束大部分结束时，我们可以获得超过20万的Bombyx蛾大脑。因此，我们仍然是建立离子交换色谱法作为Bombyx SMPH活性物质的纯化步骤的重要问题

项目成果

Fujimoto, Y., Endo, K., Watanabe, M. and Kumagai, K.: "Species-specificity in the action of big and small prothoracico-tropic hormone of four species lepido-pteran insects, Mamestra brassicae, Bombyx mori, Papilio xuthus Polygonia c-aureum." Zool. Sci.8.

Fujimoto, Y.、Endo, K.、Watanabe, M. 和 Kumagai, K.：“四种鳞翅目昆虫（Mamestra brasicae、Bombyx mori、Papilio）的大小前胸激素作用的物种特异性

遠藤克彦: "昆虫の脱皮と変態のタイミングを決めるホルモン." 化学と工業. 43. 36-40 (1990)

Katsuhiko Endo：“决定昆虫蜕皮和变态时间的激素。化学与工业”43. 36-40 (1990)。

Nakaoka, S. and Endo, K.: "Entrainment of the Pro-Thoracico-Tropic Hormone (PTTH) secretion in the swallowtail, Papilio xuthus L. (Lepidoptera : Papilionidae)." Appl. Ent. Zool.25. 95-104 (1990)

Nakaoka, S. 和 Endo, K.：“凤蝶 Papilio xuthus L.（鳞翅目：凤蝶科）中促胸热带激素 (PTTH) 分泌的夹带。”

Endo, K.: "Insect hormones determining the timing of ecdysis and metamorphosis." Kagaku to Kogyo. 43. 36-40 (1990)

Endo, K.：“昆虫激素决定蜕皮和变态的时间。”

Endo, K.: "Hormones determining the seasonal morphs of the butterflies." Insect Physiology ed, Ohnishi et al., Asakura Press. Tokyo. 24-44 (1990)

Endo, K.：“激素决定蝴蝶的季节性变形。”