Gene-engineered hybrid prostheses for small vascular reconstruction

用于小血管重建的基因工程混合假体

• 批准号: 14380387
• 负责人: HIRATA Satoshi
• 金额: $ 4.86万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14380387/
• 关键词: small caliber proshteses; hybrid artificial graft; gene transfer; alloeraft; decellularized graft; HVJ; HCG; intimal hyperplasia; 血管新生; 内皮細胞; 肝細胞増殖因子HGF; HGF(肝細胞増殖因子); 人工血管; 小口径; hepatocyte growth factor; 平滑筋細胞

Difficulty of the healing process between human being and artificial materials is main cause of graft failure of artificial arterial prostheses in small caliber arterial reconstruction. This research project has been perform in order to overcome this problem and establish small caliber prostheses.To resolve this difficult situation, we perform the following research and try to 1)establish of gene transfer methods for vascular cells, 2)developement of small caliber arterial graft.1)Establish of gene transfer methods for vascular wall cells : We perform gene transfer to blood vessel by three different methods including pressure method, HVJ-Envelope vector method, and ultrasonic method, and evaluate the transfer efficiency and degree of structural damage of vessel wall. As the results of this research, the HVJ-Envelope method is most effective and less invasive for gene transfer to vessel wall cells.2)Development of small caliber prostheses : smooth muscle cell seeding hybrid artificial p … More rostheses was evaluated in vitro as well as in vivo. Human cell growth as well as cell repopulation on the artificial material is much inferior compared to other species, even if growth factors supply or hepatic growth factor gene transfer. Anti-thrombotic characteristics is also unsolved problem for small caliber artificial prostheses. Therefore, hybrid artificial grafts were all occluded in canine femoral arterial reconstruction model. On the other hands, biologic materials such as arterial allograft have superior characteristics regarding antithrombogenecity and cell repopulation.In conclusion, it is still difficult to establish small caliber artificial prostheses because of human system against artificial materials. However, the biological materials such as allograft have promising characteristics in terms of anti-thrombogenicity and cell repopulation. So, tissue engineered allograft such as decellularized allograft or gene transferred allograft are next target of small caliber prostheses. Less

人类与人造材料之间的愈合过程困难是小口径动脉重建中人工动脉假体移植失败的主要原因，本研究项目的目的是克服这一问题并建立小口径假体。 ，我们进行了以下研究并尝试1）建立血管细胞的基因转移方法，2）开发小口径动脉移植物。1）建立血管壁细胞的基因转移方法：我们通过压力法、HVJ-Envelope载体法和超声波法三种不同的方法将基因转移到血管，并评估转移效率和血管壁的结构损伤程度作为本研究的结果，HVJ-信封方法对于将基因转移到血管壁细胞而言是最有效且侵入性较小的。2)小口径假体的开发：平滑肌细胞播种混合人工假体在体外和体内对人体细胞生长进行了评估。由于人工材料上的细胞再生能力比其他物种差很多，即使有生长因子供应或肝生长因子基因转移，抗血栓特性对于小口径人工假体来说也是未解决的问题，因此混合型人工移植物均被封闭。另一方面，在犬股动脉重建模型中，同种异体动脉等生物材料在抗血栓形成和细胞再生方面具有优越的特性。然而，同种异体移植物等生物材料在抗血栓形成和细胞再增殖方面具有广阔的前景，因此，脱细胞同种异体移植物或基因转移同种异体移植物等组织工程同种异体移植物是下一个目标。小口径假体的目标较少。