Analysis of Mucosal Immunity by the Fc receptor for IgA and IgM

IgA 和 IgM Fc 受体的粘膜免疫分析

• 批准号: 14207024
• 负责人: SHIBUYA Akira
• 金额: $ 27.62万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-14207024/
• 关键词: Fcα; μ receptor; IgA; IgM; Paneth cell; Antibacteria; Fc receptor; 自然免疫; 獲得免疫; 免疫グロブリン; 生体防御

We previously identified an Fc receptor for IgA and IgM (Fcα/μ receptor), which is expressed on antigen presenting cells. We found that the Fcα/μ receptor mediated endocytosis of IgM immune complex with antigen, such as staphylococcus aureus, suggesting an important mechanisms of pathogen eradication by IgM. The present study aims to clarify a role of the Fcα/μ receptor in mucosal immunity. We have identified several isoforms of the soluble Fcα/μ receptor expressed on intestinal Paneth cells and epithelial cells of urinary tract. We found that the extracellular domain of the isoforms may have antimicrobial activity against E.coli. Furthermore, we observed that mice deficient in the Fcα/μ receptor gene exhibited high IgA concentration in both serum and fecus and the presence of many IgA producing cells in lamina proplia.We also observed that the Fcα/μ receptor was strongly expressed on the area of germinal center. Immunohistochemical study revealed that FDC substantially expressed the Fcα/μ receptor in germinal center. These results suggest that the Fcα/μ receptor regulates IgA production in peyel's patches and/or intestinal flora by the soluble Fcα/μ receptor secreted from Paneth cells. Furthermore, we studied the mucosal immune responses by antigen challenge in mice deficient in the the Fcα/μreceptor gene.

我们先前鉴定出在抗原呈递细胞上表达的IgM（FCα/μ受体）的FC受体研究旨在阐明在肠道细胞和尿路上皮细胞上表达的可溶性FCα/μ受体的FCαLs型/μ受体基因在血清和粪便中均表现出高IgA浓度，并且在层中存在许多Iga产生细胞。我们还观察到Fcα/μ受体在生发中心表达了FCα/μ受体。这些结果中的μ受体表明，FCα/μ受体调节Peyel斑块中的IgA产生，或者通过paneth细胞的可溶性分泌的肠道菌群在抗原挑战中被抗原挑战。

项目成果

Treatment with alpha-galactosylceramide attenuates the development of bleomycin-induced pulmonary fibrosis.

• 发表时间: 2004
• 期刊: Journal of immunology
• 影响因子: 4.4
• 作者: Toru Kimura;Yukio Ishii;Y. Morishima;A. Shibuya;K. Shibuya;M. Taniguchi;M. Mochizuki;A. Hegab;T. Sakamoto;A. Nomura;K. Sekizawa

Iwama, A. et al.: "CCAT/enhancer binding protein(C/EBP) and PU. 1 transcriptoin factors in Language cell commitment"Journal of Experimental Medicine. 195. 547-558 (2002)

Iwama, A. 等人：“语言细胞定型中的 CCAT/增强子结合蛋白 (C/EBP) 和 PU.1 转录因子”实验医学杂志。

Kojima H., Shibuya A.et al.: "CD226 mediates platelet and megakaryocytic cell adhesion to vascular endothelial cells"J.Biol.Chem.. 278・367. 48-53 (2003)

小岛H.、涩谷A.等：“CD226介导血小板和巨核细胞与血管内皮细胞的粘附”J.Biol.Chem.. 278・367(2003)。

Successful gene transfer into human CD4 positive T cells mediated by lentiviral vectors.

由慢病毒载体介导成功地将基因转移到人类 CD4 阳性 T 细胞中。

• 发表时间: 2004
• 期刊: Immunology 12
• 作者: Shibuya K;et al.
• 通讯作者: et al.

Requirement of the tyrosines at residues 258 and 270 of MAIR-1 in inhibitory effect on degranulation from basophilic leukemia RBL-2H3.

MAIR-1 残基 258 和 270 处的酪氨酸对嗜碱性白血病 RBL-2H3 脱粒的抑制作用的需要。

• 发表时间: 2005
• 期刊: International Immunology 17
• 作者: Suto A;Nakajima H;Takatori H;Tokumasa N;Suzuki K;Iwamoto I;Shirakawa J et al.;Shibuya A et al.;Okoshi Y et al.
• 通讯作者: Okoshi Y et al.