A study of the membrane-bound factors that underlie the formation of thalamocortical projections

丘脑皮质投射形成背后的膜结合因子的研究

• 批准号: 13680870
• 负责人: YAMAMOTO Nobuhiko
• 金额: $ 2.18万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13680870/
• 关键词: neocortex; thalamus; Eph receptor; neurotrophin; axonal growth; axonal guidance; 大脳皮質; ephrin-B3; 軸索誘導; 遺伝子発現; 層特異性; 領野; ラット

The thalamocortical projection is one of the interesting systems to study the mechanisms that underlie generation of specific neuronal connections in the brain. During development, thalamic axons in grow into the cortex, which is composed of six cell layers, and selectively project to layer 4, the target layer. To date, we have demonstrated that axonal stopping and branching are crucial behavior for the targeting mechanism, by using organotypic coculture preparations. In this study we study the role of the membrane-associated and diffusible molecules to thalamic axon growth and guidance. The result suggests that ephrin-B3contributes to regional and layer specificity by suppressing axonal growth from lateral thalamic neurons. Moreover, evidence suggest that neurotrophins promote axonal growth in a substratum-dependent manner in the development of thalamocortical connections.

丘脑皮层投影是研究大脑中特定神经元连接的机制的有趣系统之一。在发育过程中，丘脑轴突中的轴突生长成由六个细胞层组成的皮层，并有选择地投射到第4层（目标层）。迄今为止，我们已经证明了通过使用器官型共培养准备，轴突停止和分支是靶向机制的关键行为。在这项研究中，我们研究了膜相关和扩散分子对丘脑轴突生长和指导的作用。结果表明，通过抑制丘脑外侧神经元的轴突生长，ephrin-b3贡献了区域和层特异性。此外，有证据表明，神经营养蛋白在丘脑皮质连接的发展中以底层依赖性方式促进轴突生长。

项目成果

Yamamoto, N.: "Wiring of the brain by a range of guidance cues"Prog. Neurobiol. (in press). (2003)

Yamamoto, N.：“通过一系列引导线索连接大脑”Prog。

Yamamoto, N.: "Wiring of the brain by a rang of guidance cues."Prog. Neurobiol.. 68. 393-407 (2003)

Yamamoto, N.：“通过一系列引导线索连接大脑。”Prog。

Takemoto, N.: "Role of ephrin-B3 in the formation of region- and lamina-specific thalamocortical projections."Eur. J. Neurosci.. 16. 1168-72 (2002)

Takemoto, N.：“肝配蛋白-B3 在区域和层特异性丘脑皮质投射形成中的作用。”Eur。

Molnar, Z.: "Development of functional thalamocortical synapses studied with current source-density analysis in whole forebrain slices in the rat"Brain Res. Bull. (in press). (2003)

Molnar，Z.：“利用大鼠整个前脑切片的电流源密度分析研究功能性丘脑皮质突触的发育”Brain Res。

Yamamoto, N.: "Cellular and molecular basis of axonal targeting in the formation of lamina-specific thalamocortical projections."Neurosci Res. 42. 167-173 (2002)

Yamamoto, N.：“形成椎板特异性丘脑皮质投射的轴突靶向的细胞和分子基础。”神经科学研究。