Immunohistlogical study of mouse periodontal burst model

小鼠牙周爆裂模型的免疫组织学研究

基本信息

批准号：
13672192
负责人：
HARA Yoshitaka
金额：
$ 2.3万
依托单位：
NAGASAKI University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672192/
关键词：
LPS Bone resorption Interferron-γ IL-1 β Osteoclast LPS Interferon-γ IL-1β

项目摘要

It has been reported that bacterial lipopolysaccharide (LPS) induces alveolar bone resorption and that the host immune system especially activated T cells, plays a crucial role in osteoclastogenesis. On the other hand, interferon-gamma (IFN-γ), which is produced by activated T cells, suppresses bone resorption both in vitro and in vivo. Thus, the question arises as to whether or not IFN-γ production increases with increasing bone resorption. We previously demonstrated that repeated injections of Escherichia coli LPS into mouse gingiva causes osteoclast formation in alveolar bone. In the present study we observed changes in the IFN-γ production of infiltrating cells in concurrence with bone resorption. Mice were repeatedly injected with 5μg LPS 26times every 48h. After the 16th injection when the alveolar bone resorption reached a plateau, the concentration of LPS was altered (25μg LPS or PBS alone). The level of active resorption became significantly elevated, and the number of IFN-γ- and interleukin-1 beta (IL-1 β)- bearing cells also increased significantly, in relation to bone resorption within the 25γg LPS injected group. On the other hand, few tartrate-resistant acid phosphatase positive cells, or IFN-γ- and IL-1 β- bearing cells, were seen in the PBS injected group. These results suggest that alteration in IFN-γ-bearing cells might play arole in counterbalancing LPS induced bone resorption resulting from osteoclast activating cytokines such as IL-1 β.

据报道，细菌脂多糖（LPS）诱导肺泡骨溶解，宿主免疫系统尤其是激活的T细胞，在破骨细胞生成中起着至关重要的作用。另一方面，由活化的T细胞产生的干扰素 - γ（IFN-γ）抑制了体外和体内的骨骼分辨率。这是关于IFN-γ产生是否随着骨骼分辨率增加而产生的问题。我们先前证明，将大肠杆菌LPS重复注射到小鼠牙龈中会导致牙槽骨的破骨细胞形成。在本研究中，我们观察到浸润细胞与骨骼分辨率一致的IFN-γ产生的变化。每48h每48h每48h反复注射5μgLps。第16次注射后，当肺泡骨溶解达到平稳时，LPS的浓度被改变（仅25μgLPS或PBS）。活动分解的水平显着升高，与注射的25γGLPS内的骨骼分辨率有关，IFN-γ-和白介素-1β（IL-1β） - 轴承细胞的数量也显着增加。另一方面，在注射的PBS组中可以看到很少有耐锈酸酸性磷酸酶阳性细胞，或IFN-γ-和IL-1β-轴承细胞。这些结果表明，含有IFN-γ的细胞的改变可能会在平衡LPS引起的骨骼分辨率中发挥作用，这是由破骨细胞激活细胞因子（如IL-1β）引起的。