Immunohistlogical study of mouse periodontal burst model

小鼠牙周爆裂模型的免疫组织学研究

基本信息

批准号：
13672192
负责人：
HARA Yoshitaka
金额：
$ 2.3万
依托单位：
NAGASAKI University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672192/
关键词：
LPS Bone resorption Interferron-γ IL-1 β Osteoclast LPS Interferon-γ IL-1β

项目摘要

It has been reported that bacterial lipopolysaccharide (LPS) induces alveolar bone resorption and that the host immune system especially activated T cells, plays a crucial role in osteoclastogenesis. On the other hand, interferon-gamma (IFN-γ), which is produced by activated T cells, suppresses bone resorption both in vitro and in vivo. Thus, the question arises as to whether or not IFN-γ production increases with increasing bone resorption. We previously demonstrated that repeated injections of Escherichia coli LPS into mouse gingiva causes osteoclast formation in alveolar bone. In the present study we observed changes in the IFN-γ production of infiltrating cells in concurrence with bone resorption. Mice were repeatedly injected with 5μg LPS 26times every 48h. After the 16th injection when the alveolar bone resorption reached a plateau, the concentration of LPS was altered (25μg LPS or PBS alone). The level of active resorption became significantly elevated, and the number of IFN-γ- and interleukin-1 beta (IL-1 β)- bearing cells also increased significantly, in relation to bone resorption within the 25γg LPS injected group. On the other hand, few tartrate-resistant acid phosphatase positive cells, or IFN-γ- and IL-1 β- bearing cells, were seen in the PBS injected group. These results suggest that alteration in IFN-γ-bearing cells might play arole in counterbalancing LPS induced bone resorption resulting from osteoclast activating cytokines such as IL-1 β.

据报道，细菌脂多糖（LPS）可诱导牙槽骨吸收，而宿主免疫系统（尤其是活化的 T 细胞）在破骨细胞生成中起着至关重要的作用。激活的 T 细胞在体外和体内均抑制骨吸收。因此，我们之前证明重复注射 IFN-γ 是否会随着骨吸收的增加而增加。将大肠杆菌LPS注入小鼠牙龈会导致牙槽骨中破骨细胞的形成，在第16次注射后，我们观察到浸润细胞的IFN-γ产生与骨吸收同时发生的变化。当牙槽骨吸收达到平台时，改变 LPS 的浓度（25μg LPS 或 PBS与25γg LPS注射组内的骨吸收相关，主动吸收水平显着升高，并且IFN-γ-和白细胞介素-1β（IL-1β）-携带细胞的数量也显着增加。另一方面，在 PBS 注射组中观察到很少的抗酒石酸酸性磷酸酶阳性细胞或携带 IFN-γ 和 IL-1 β 的细胞。携带 IFN-γ 的细胞可能在平衡由破骨细胞激活细胞因子（如 IL-1β）引起的 LPS 诱导的骨吸收中发挥作用。