Experimental study of the effect of T cell on alveolar bone resorption induced by bacterial endotoxin

T细胞对细菌内毒素诱导的牙槽骨吸收影响的实验研究

基本信息

批准号：
05454519
负责人：
HARA Yoshitaka
金额：
$ 4.54万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (B)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05454519/
关键词：
Bone resorption T cell Nude mouse SCID mouse T細胞病理組織学的検索

项目摘要

Periodontal disease are caused by bacterial infection and some immune cells locally infiltrated against it. There cells secrete cytokines which have been reported to act not only as regulatory substances for immune responses, but mediators of tissue destruction. In fact, there have been many reports which suggest the relation between some cytokines secreted from T cell, a mediator of immune responses, and alveolar bone resorption. However, it has been unclear that T cells truly relate with periodontal tissue destruction. So in first study, alveolar bone resorption induced by every 48 h injections of E.coli endotoxin was investigated in normal mice, T cell deficient nude mice and SCID mice congenitally lacking both T and B cells. In addition, CD4 and/or CD8 depleted mice by the administration of anti-CD4 and/or anti-CD8 monoclonal antibodies were used in this study. In normal mice, alveolar bone resorption was observed after 4 times injection, and after 7 times in nude mice and 10 times in SCID mice. T cell depleted mice, especially CD4 depleted mice, also revealed the weaker and delayd bone resorption. These findings strongly suggested the relation between T cells and bone resorption. So in second experiment, mice transfered with T cells were established and injected with endotoxin as same as in first study. After 4 times injection, these mice showed the weak but obvious bone resorption similarly in normal mice. Based on these results, T cells closely related the bone resorption induced by endotoxin and further study should be investigated on cytokine production by T cells.

牙周疾病是由细菌感染引起的，一些免疫细胞局部浸润。细胞分泌细胞因子，据报道，不仅充当免疫反应的调节物质，而且是组织破坏的介质。实际上，有许多报道表明，从T细胞分泌的一些细胞因子，免疫反应的介体和肺泡骨吸收之间的关系。但是，尚不清楚T细胞与牙周组织破坏真正相关。因此，在第一项研究中，在正常小鼠，T细胞缺乏裸鼠和SCID小鼠中，研究了每48 h注射大肠杆菌内毒素每48 h注射肺泡骨吸收。此外，在这项研究中使用了抗CD4和/或抗CD8单克隆抗体的CD4和/或CD8耗尽小鼠。在正常小鼠中，注射4次后观察到肺泡骨吸收，在裸鼠中，在SCID小鼠中观察到7次。 T细胞耗尽的小鼠，尤其是CD4耗尽的小鼠，也揭示了较弱和延迟的骨吸收。这些发现强烈建议T细胞与骨吸收之间的关系。因此，在第二个实验中，建立了用T细胞转移的小鼠并注射内毒素与第一研究一样。注射4次后，这些小鼠在正常小鼠中表现出较弱但明显的骨吸收。基于这些结果，T细胞与内毒素诱导的骨吸收密切相关，并应研究T细胞的细胞因子产生。