Development of functionally graded DNA films containing cytokines eliciting bone formation

含有诱导骨形成的细胞因子的功能分级 DNA 膜的开发

• 批准号: 13672070
• 负责人: FUKUSHIMA Tadao
• 金额: $ 2.62万
• 依托单位: Fukuoka Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672070/
• 关键词: DNA; Lipid; Cytokine; DNA film; DNA-lipid complex; Antibacterial activity; Intercalation; Tensile strength

The DNA-lipid complexes with and without cytokines BMP and b-FGF were prepared synthetic lipids from the reaction of n-alkyl alcohol (C=4〜C=20) and amino acids (glycine, L-alanine and L-glutamic acids) and the DNA films were obtained by casting the DNA -lipid complexes from chloroform/ethanol solution. The tensile strengths and intercalation behavior of DNA films were investigated. Antibacterials characteristics of DNA-lipid complexes against bacteria (P. gingivalis, S. mutans, S. aureus and P. aeruginosa) were also investigated. Additionally, effect of the DNA -lipid complexes with cytokines BMP and b-FGF on bone formation was evaluated. The DNA-lipid films had tensile strengths of approximately 2MPa, and formed intercalation complexes with ethidium bromide. The formation of the intercalation complex of DNA-lipid film with ethidium bromide was influenced by the length of the alkyl chains of the synthesized lipids. The complexes prepared from DNA with glycine decyl ester or glycine dodecyl ester, and with L-alanine decyl ester or glycine dodecyl ester showed antibacterial activity. However, the DNA-lipid complexes with cytokines BMP and b-FGF did not elicit bone formation.

含有和不含有细胞因子BMP和b-FGF的DNA-脂质复合物是由正烷基醇(C=4〜C=20)和氨基酸(甘氨酸、L-丙氨酸和L-谷氨酸)反应制备的合成脂质采用氯仿/乙醇溶液浇铸DNA-脂质复合物得到DNA薄膜，并研究了DNA-脂质复合物的拉伸强度和嵌入行为。此外，还研究了 DNA-脂质复合物与细胞因子 BMP 和 b-FGF 对细菌（牙龈卟啉单胞菌、变形链球菌、金黄色葡萄球菌和铜绿假单胞菌）的作用。拉伸强度约为2MPa，与溴化乙锭形成插层复合物。DNA-脂质膜与溴化乙锭的插层复合物的形成受长度的影响。由DNA与​​甘氨酸癸酯或甘氨酸十二烷基酯以及与L-丙氨酸癸酯或甘氨酸十二烷基酯制备的复合物显示出抗菌活性，然而，DNA-脂质与细胞因子BMP和b的复合物显示出抗菌活性。 -FGF不引起骨形成。