Pathogen enzyme productivity of bacteria isolated from a therapy resistant periapical periodontitis cases

从治疗耐药的根尖周炎病例中分离出的细菌的病原体酶生产力

• 批准号: 13672017
• 负责人: YOSHIDA Masahiro
• 金额: $ 0.9万
• 依托单位: Osaka Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672017/
• 关键词: therapy-restant periodontis; Enterococcs faecalis; Pseudomonas aeruginosa; Streptococcus oralis; Pathogen enzyme productivity; chondroitin sulfatase; collagenase; hyaluronidase; Candida; 組織侵襲性; 病原性; チェアーサイド嫌気培養システム; 抗菌薬局所投与; Candida albicans

Refractory periapical periodontitis is caused by persistent infection by bacteria to resist for endodontic treatment such as Enterococcs faecalis, Candida albicans, Pseudomonas aeruginosa and Streptococcus oralis.Many of refractory periapical periodontitis cases are subacute cases, but those symptom is various.The bacteria which resisted therapy examined productivity in pathogen enzyme for the purpose of it was clear, and in this study, these doing the effect which therapy resistant bacteria gave to clinical manifestation expression of a refractory periapical periodontitis case.The type strain (ATCC35039) and the test strain of S.oralis did hot show pathogen enzyme production characteristics.E.faecalis showed chondoroitin sulfatase producing.P.aeruginosa showed lecithinase producing and weak producing such as collagenase, hyaluronidase and chondoroitin sulfatase in high frequency.These resultant showed the probability which pathogen enzyme production characteristics of these bacteria participated in refractory periapical periodontitis developing for clinical symptom persistently.

难治性根尖周炎是由粪肠球菌、白色念珠菌、铜绿假单胞菌、口腔链球菌等抵抗牙髓治疗的细菌持续感染引起的。难治性根尖周炎病例多为亚急性病例，但症状多种多样。抵抗治疗的细菌检查病原体酶的生产力的目的是明确的，并且在此研究表明，治疗耐药菌对难治性根尖周炎病例临床表现的影响。口链球菌的标准菌株（ATCC35039）和测试菌株确实显示出病原体酶的产生特征。粪肠球菌显示出软骨素硫酸酯酶铜绿假单胞菌表现出产卵磷脂酶和弱产胶原酶、透明质酸酶、软骨素硫酸酯酶等这些结果显示了这些细菌的病原酶产生特征参与了难治性根尖周炎持续发展为临床症状的可能性。

项目成果

吉田 匡宏: "難治症例を治す"歯科医学. 64(1). 66-71 (2001)

Masahiro Yoshida：“治疗疑难病例”牙科 64(1) (2001)。