No Invasive Methods For Cell Proliferation Activity Using Superconduction Quantum Interference Device

利用超导量子干涉装置进行细胞增殖活性的无创方法

基本信息

批准号：
13671530
负责人：
KIM Wook-cheol
金额：
$ 1.09万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671530/
关键词：
SQUID cell proliferation micro electoromagnetic fields tissue maturation SQUID 細胞増殖微小電位

项目摘要

We tried to measure a electoromagnetic field changes with cell proliferation using Superconduction Quantum Interference Device in 2001. A lot of noise and base line shift made disturbance of detection of the micro electromagnetic field changes, becouse air circulating system to keep optimal temperature for cell proliferation made the test condition worse. Therefore, we took a long time to get the good condition in order to detect the micro electoromagnetic field changes with cell proliferation. However, we could not get the optimal conditions for this test system. Next year we tried to measure the electlomagnetic field changes with cell proliferation under room temperature. The noise and base line shift was reduced. However, we could not detect remarkable changes. Unfortunately, mono channel censer of SQUID could not be available to our research, then we applied to use the multi channel censers for brain activity. Now we will try to calibrate the electromagnetic fields for our study in near futureWe also continued to research for the impedance of bone tissue in the process of callus formation in order to detect tissue level activity. We used lengthend callus of tibiae in rabbits. The waiting period was 1 week and the lengthening rate was 0.5mm twice daily. The impedance values of lengthened tissue increased with the callus maturation. Impedance was 5.41 ^^+__- 1.75 KΩ. It was useful data as normal callus impedance in order to compare to malignant bone tumor tissue

2001年我们尝试用超导量子干涉仪测量电磁场随细胞增殖的变化。大量的噪音和基线偏移对微电磁场变化的检测造成干扰，因为空气循环系统保持细胞增殖的最佳温度使得因此，我们花了很长时间才获得良好的条件来检测微电磁场随细胞增殖的变化，但我们无法获得该测试的最佳条件。明年，我们尝试测量室温下细胞增殖时的电磁场变化，但我们无法检测到显着的变化。研究，然后我们申请使用多通道香炉来进行大脑活动，现在我们将在不久的将来尝试校准我们的研究的电磁场我们还继续研究骨组织在骨痂形成过程中的阻抗。为了检测组织水平的活性，我们使用了兔子胫骨的延长骨痂，等待时间为1周，延长率为0.5mm，每天两次，随着骨痂成熟，延长的组织的阻抗值为5.41。 ^^ +__- 1.75 KΩ 这是作为正常愈伤组织阻抗的有用数据，以便与恶性骨肿瘤组织进行比较。