Profile analysis of gene expressions for cereberal vasospasm

脑血管痉挛基因表达谱分析

• 批准号: 13671472
• 负责人: KASUYA Hidetoshi
• 金额: $ 1.98万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671472/
• 关键词: subarachnoid hemorrhage; cerebral vasospasm; gene expression

Introduction : We sought to identify genes with differential expression in cerebral vasospasm after subarachnoid hemorrhage. This study was undertaken to identify hemolysate-induced differential gene expression in human vascular smooth muscle cells (HVSMC) with cDNA microarray (12814 genes).Methods : Total ribonucleic acid (RNA) was isolated from hemolysate treated and non-treated HVSMC. Total RNAs were used to quantify lesion-specific mRNA expression levels on cDNA microarray (days 0-5). The expression of 30 genes greater than 4 fold with cDNA microarray was confirmed with real-time RT-PCR (days 0-5).Result : There was significant up-regulation (greater than 2 fold expression) of 397 genes in the hemolysate treated cells on day 1, 49 genes on day 2, 23 genes on day 3, 31 genes on day 5. The functions of differentially expressed genes included the regulation of inflammation, membrane proteins and receptors, kinase, cell proliferation, and phosphatases. 11924 genes did not change significantly (0.5-2 fold) or did not express, and 74 genes were down-regulated (lower than 0.5 fold) on day 2. The expressions of inflammatory cytokines/chemokines such as interleukin(IL)-8, IL-1β were extremely increased on day 2, followed by gradual decline when measured with real-time RT-PCR serially.Conclusion : We identify numerous genes that are differentially expressed in control and hemolysate-treated cells. The up-regulation of genes related to inflammatory cytokines/chemokines and its time course in HVSMC suggest that they may play a significant role in vasospasm.

简介：我们感觉到在蛛网膜下腔出血后识别脑血管痉挛中具有差异表达的基因。进行了这项研究，以鉴定出使用cDNA微阵列（12814个基因）的人血管平滑肌细胞（HVSMC）中的血溶剂诱导的差异基因表达。方法：总核糖核酸（RNA）是从溶血处理和未培养的HVSMC中分离出来的。总RNA用于定量cDNA微阵列上病变特异性的mRNA表达水平（第0-5天）。用实时RT-PCR（第0-5天）证实了30个基因的表达大于4倍的30个基因的表达。分辨率：在第1天，第1天，第1天，第3天，第3天，第3天，第5天的310基因在第5天的炎症中，有明显的上调（大于2倍表达）的397个基因在第1、49天的基因。受体，激酶，细胞增殖和磷酸酶。 11924个基因在第2天没有显着变化（0.5-2倍）或未表达，并且74个基因在第2天下降（低于0.5倍）。炎性细胞因子/趋化因子/趋化因子/趋化因子的表达（例如，介入量表（IL）-8，IL-8，IL-1β在第2天都极大地增加了，随后是循环，并在实时RT-PCR中进行了多种表达，并在实时RT-PCR上进行了多种测量。溶血治疗的细胞。与炎性细胞因子/趋化因子有关的基因的上调及其在HVSMC中的时间过程表明它们可能在血管痉挛中起重要作用。

项目成果

Yoneyama T, Kasuya H, Onda H, et al.: "Association of positional and functional candidate genes FGF1, FBN2, and LOX on chromosome 5q31 with intracranial aneurysm."J Hum Genet. 48. 309-314 (2003)

Yoneyama T、Kasuya H、Onda H 等人：“染色体 5q31 上位置和功能候选基因 FGF1、FBN2 和 LOX 与颅内动脉瘤的关联。”J Hum Genet。

Yoneyama T, Kasuya H, Onda H, et al.: "Collagen Type Iα2 (COL1A2) Is the Susceptible Gene for Intracranial Aneurysms"Stroke. 35. 443-448 (2004)

Yoneyama T、Kasuya H、Onda H 等人：“Iα2 型胶原蛋白 (COL1A2) 是颅内动脉瘤的易感基因”35. 443-448 (2004)

Kasuya H, Onda H, Yoneyama T, et al.: "Bedside Monitoring of Circulating Blood Volume After Subarachnoid Hemorrhage"Stroke. 34. 956-960 (2003)

Kasuya H、Onda H、Yoneyama T 等人：“蛛网膜下腔出血后循环血量的床边监测”中风。

Hiyama H, Kasuya H, Dan S, et al.: "Orbital syndrome after a surgery of vestibular schwannoma via middle fossa approach"Acta Neurochir (Wien). 145. 83-85 (2003)

Hiyama H、Kasuya H、Dan S 等人：“通过中颅窝入路进行前庭神经鞘瘤手术后的眼眶综合征”Acta Neurochir（维也纳）。

Kasuya et al: "Development of nicardipine prolougest-release implants for preventing vasospasm"Acta Neurochir[Suppl]. 77. 217-220 (2001)

Kasuya 等人：“用于预防血管痉挛的尼卡地平缓释植入物的开发”Acta Neurochir[增刊]。