Expression of endothelial nitric oxide synthase and soluble guanylate cyclase in the arterial wall after SAH

SAH后动脉壁内皮一氧化氮合酶和可溶性鸟苷酸环化酶的表达

• 批准号: 06671424
• 负责人: KASUYA Hidetoshi
• 金额: $ 1.34万
• 依托单位: Tokyo Women's Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671424/
• 关键词: SAH; cerebral vasospasm; nitric oxide synthase; soluble guanylate cyclase; Western blot; Northern blot; endothelin; transforming growth factor beta1; カルポニン; くも膜下出血

Endothelium-dependent vasodilations are impaired during cerebral vasospasm following subarachnoid hemorrhage. The alterations of endothelial nitric oxide synthase (eNOS) and soluble guanylate cyclase (sGC) were determined by Western and Northern blot analysis in the canine basilar artery of double hemorrhage model. Thirty dogs were assigned to three groups : Group D0=control ; D2=dogs sacrificed 2 days after cisternal injection of blood ; D7=dogs with double cisternal injection of blood sacrificed 7 days after the first one. The extract from basilar arteries contained two bands, 135 kDa specific for eNOS and 120 kDa, which may be a existance of degradation product of eNOS.The densitometer values were presented as percentages of D0 control value. Although the signal in D7 group was less than that of the D0 control, it was not statistically significant. A single major band corresponding to alpha-subunit of sGC was seen at 70 kDa in the extract from basilar arteries. The signals of D2 and D7 were 69<plus-minus>40%, and 25<plus-minus>18%(p<0.001), respectively. The reduced expression of sGC may be related to the impairment of endothelium-dependent vasodilation. Northern blot analysis failed to demonstrate signals of eNOS and sGC using 10 mug of total RNA extracted from the canine basilar arter

蛛网膜下腔出血后脑血管痉挛期间，内皮依赖性血管舒张受损。在双出血模型的犬基底动脉中，通过西部和北印迹分析确定内皮一氧化氮合酶（ENOS）和可溶性鸟烯酸酸酯环化酶（SGC）的改变。将三十只狗分配给三组：D0组=对照； d2 =血液塞斯特尔注射血液后2天处死的狗； D7 =第一个狗在第一个狗的7天后牺牲了双脑胞质注射的狗。来自基底动脉的提取物包含两个频段，特定于eNOS和120 kDa，这可能是eNOS的降解产物的存在。密度计值以D0控制值的百分比表示。尽管D7组中的信号小于D0对照的信号，但在统计学上并不显着。从基底动脉提取物中，可以看到与SGCα-亚基相对应的单个主要条带。 D2和D7的信号分别为69 <plus-minus> 40％和25 <plus-minus> 18％（p <0.001）。 SGC的表达降低可能与内皮依赖性血管舒张的损害有关。 Northern印迹分析未能使用从犬基底动脉提取的10杯总RNA证明eNOS和SGC的信号

项目成果

Kasuya H,Weir B,Ghahary A: "Transforming growth factor b1, and procollagen types I and III gene expression of canine basilar artery in vasospasm." Neurosurgery. (submitted).

Kasuya H、Weir B、Ghahary A：“血管痉挛时犬基底动脉的转化生长因子 b1 以及 I 型和 III 型前胶原基因表达。”

糟谷英俊: "クモ膜下出血後脳血管内皮型NO合成酵素と可溶性グアニレートサイクラーゼ発現の変化" 脳血管攣縮. 10. 2-9 (1995)

Hidetoshi Kasuya：“蛛网膜下腔出血后脑血管内皮NO合酶和可溶性鸟苷酸环化酶表达的变化”脑血管痉挛。 10. 2-9 (1995)

糟谷英俊: "oxyhemoylobinのendothelin産成刺激作用" 脳血管攣縮. 9. 267-271 (1994)

Hidetoshi Kasuya：“氧合血红蛋白对内皮素产生的刺激作用”脑血管痉挛。 9. 267-271 (1994)

H.Kasuya et al: "Nitric oxide Synthase and guanylute cyclase levcls in Canine basilar artery after SAH" J.Neurosurg. 82. 250-255 (1995)

H.Kasuya 等人：“SAH 后犬基底动脉中的一氧化氮合酶和鸟苷酸环化酶水平”J.Neurosurg。

Kasuya: "Nitric oxide synthase and guahylate cyclase levels in canine basilar artery after SAH" J. Neurosurg. 82. 250-255 (1995)

Kasuya：“SAH 后犬基底动脉中一氧化氮合酶和胍基乙酸环化酶的水平”J. Neurosurg。