Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin V

使用心肌膜联蛋白 V 单克隆抗体检测阿霉素诱导的心肌细胞损伤

• 批准号: 13670969
• 负责人: KOBAYASHI Hideki
• 金额: $ 1.22万
• 依托单位: Tokyo Women's Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670969/
• 关键词: anti-Annexin V antibody; imaging; 抗体イメージング; アドリアマイシン心筋症; 早期発見; アドリアマイシン心筋症モデル; イメージング

Detection of Doxirubicin Induced Mycardial cell injury using Monoclonal Antibodies for Myocardial Annexin VAnnex in V, a calcium-dependent phospholipid-binding protein that exists in the soluble fraction of myocardium, was translocated from the soluble fraction of myocardium to the myocardial cell membrane when the myocardium was damaged in the patients with congestive heart failure. The purpose of this sturdy was to clarify the diagnostic value of In-111 labeled Monoclonal Antibodies for Myocardial Annexin V for the detection of damaged myocardium. Methods. Forty Wister rats were injected 0.2mg of doxirubicin weekly for making doxirubicin induced cardiomyopathy model. In-111 labeled anti-Annexin V antibody was injected 4, 6, 8 and 9 weeks after the doxirubicin Injection. Results. Heart/Lung ratio in doxirubicin group on weeks 4, 6, 8 and 9 weeks (2.56±0.48, 2.30±0.49, 2.20±0.23, 0.85±0.41) were significantly higher than that in control group (1.30±0.21, 1.32±0.38, 1.39±0.23). Biodistribution analysis also showed that In-111 labeled anti-Annexin V antibody accumulation was higher in doxirubicin group than that in control group. Conclusion. In-111 labeled anti-Annexin V antibody scintigraphy positively imaged damaged myocardium in doxirubicin induced rat cardiomyopathy model.

使用心肌膜联蛋白 V 单克隆抗体检测阿霉素诱导的心肌细胞损伤 V 中的膜联蛋白是一种存在于心肌可溶性部分中的钙依赖性磷脂结合蛋白，当心肌膜破裂时，它从心肌可溶性部分易位至心肌细胞膜。充血性心力衰竭患者中受损。本研究的目的是阐明其诊断价值。 In-111标记的心肌Annexin V单克隆抗体用于检测受损心肌。方法40只Wister大鼠每周注射阿霉素0.2mg，制作阿霉素诱导的心肌病模型，注射4、6次。 、多柔比星注射后 8 周和 9 周的心/肺比值。阿霉素组第4、6、8、9周时（2.56±0.48、2.30±0.49、2.20±0.23、0.85±0.41）显着高于对照组（1.30±0.21、1.32±0.38、1.39±0.23）生物分布分析还表明结论 In-111标记的抗Annexin V抗体在阿霉素组中的积累高于对照组。结论。