Myocardial ischemia imaging using monoclonal antibodies

使用单克隆抗体进行心肌缺血成像

基本信息

批准号：
11670916
负责人：
KOBAYASHI Hideki
金额：
$ 1.41万
依托单位：
Tokyo Women's Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11670916/
关键词：
ischemia imaging antibodies isotope Annexin V 抗体イメージアネキシンV 心筋虚血

项目摘要

DETECTION OF MYCARDIAL ISCHHEMIC AREA USING MONOCLONAL ANTIBODIES FOR MYOCARDIAL ANNEXIN VHideki KOBAYASHI, Mitsuru MOMOSE, Kiyoko KUSAKABE, Noboru KanekoDepartment of Radiology, Tokyo Woman's Medical UniversityThe purpose of this study was to clarify the possibility of detection of myocardial ischemic area using ^<111>In labeled anti-annexin V antibody imaging. Methods Wistar rats were subjected to transient acute ischemia (10 min of left coronary occlusion and following reperfusion, ischemia group, N=31) and controls (control group, N=29). Anti-annexin V antibody labeled with 7.4 MBq of ^<111>In was injected 2, 7, 14, 21 and 50 days after the model preparation. Results Heart/lung ratio in the ischemia group on days 2, 7 and 14 (3.32±0.21, 3.03±0.2 1, 3.19±0.64) was significantly higher (p<0.01) than that in the control group (1.41±0.51, 1.14±0.36, 1.40±0.52). Fifty days after the model preparation in the ischemia group, the heart/lung ratio (2.03±0.25) was decreased compared with that in the ischemia group on days 2, 7 and 14. Macroautoradiography showed significant ^<111>In labeled anti-annexin V antibody accumulation in the left anterior descending coronary territory in the ischemia group. Microscopic examination showed no myocardial infarct area in the ischemia group. Conclusion ^<111>In labeled anti-annexin V antibody imaging clearly detected the myocardial ischemic area following 10 min of transient acute ischemia in this experimental model. Anti-annexin V antibody imaging can provide unique information regarding the ischemic memory of myocardium.

使用心肌膜联蛋白V单克隆抗体检测心肌缺血区域Hideki KOBAYASHI, Mitsuru MOMOSE, Kiyoko KUSAKABE, Noboru Kaneko东京女子医科大学放射线科本研究的目的是阐明使用^<111>In检测心肌缺血区域的可能性标记抗膜联蛋白 V方法 Wistar 大鼠进行短暂急性缺血（左冠状动脉闭塞 10 分钟，再灌注后，缺血组，N=31）和对照组（对照组，N=29）。模型制备后2、7、14、21和50天注射用7.4MBq的^ 111 In标记。结果缺血组的心/肺比。 50天后，2、7和14（3.32±0.21、3.03±0.2、3.19±0.64）显着高于对照组（p<0.01）（1.41±0.51、1.14±0.36、1.40±0.52）。缺血组模型制备、心肺比值与缺血组相比，第2、7和14天降低了(2.03±0.25)。宏观放射自显影显示缺血组左前降支区域有显着的111 In标记抗膜联蛋白V抗体积聚。结论 ^<111>在标记的抗膜联蛋白V抗体显像中清楚地检测到心肌。在该实验模型中，短暂性急性缺血 10 分钟后的缺血区域，抗膜联蛋白 V 抗体成像可以提供有关心肌缺血记忆的独特信息。