Study on Differentiation of Megakaryocytic Leukemic Cells and Differentiation Factor.

巨核白血病细胞分化及分化因子的研究。

基本信息

批准号：
01570186
负责人：
TANGE Tsuyoshi
金额：
$ 1.34万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1989
资助国家：
日本
起止时间：
1989 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-01570186/
关键词：
megakaryocytic differentiation platelet culture tyrosine kinase c-yes cーyes

项目摘要

1. Although another megakaryocytic cell line like T-33 could not be established, in our laboratory we succeeded in establishing an interesting new human myelogenous cell line derived from polycythemia vera, and an human mesothelial cell line derived from malignant mesothelioma with secondary thrombocytosis. The latter has been found to contain IL-6 and IL-3.2. When recombinant interleukin 6 (rIL-6) as a megakaryocytic differentiation factor was used to induce megakaryocytic maturation of T-33 cells, rIL-6 alone increased intracytoplasmic platelet glycoprotein (GP IIb IIIa), and also showed synergistic effect on the appearance of GP IIb IIIa on the cell surface. This indicated that rIL-6 was effective for the megakaryocytic differentiation of the cell line too.3. We continued the c-yes tyrosine protein kinase (TPK) assay for the megakaryocytic differentiation of T-33 cells, using the in vitro immuno-precipitation method. As a result of it, T-33 replatedly showed the dcereased c-yes TPK activity at 3 to 40 hours after the TPA treatment. Similarly, K562 showed the decrease at 16 to 40 hours after the same treatment. On the other hand, the monocytic cell line U937 showed the increase at 6 to 48 hours, and HL-60 cell line showed the decrease in the early phase and the subsequent increase at 36 hours. In the case of incunbation with IL-6, the TPK activity of T-33 increased at 40 hours. Contrary to the c-yes, the TPK activity of scr generally increased at 24 to 48 hours in all cell lines tested.

1.虽然无法建立另一种巨核细胞系，如T-33，但在我们的实验室，我们成功地建立了一种有趣的新的源自真性红细胞增多症的人骨髓细胞系，以及源自继发性血小板增多症的恶性间皮瘤的人间皮细胞系。后者被发现含有IL-6和IL-3.2。当重组白细胞介素6（rIL-6）作为巨核细胞分化因子诱导T-33细胞巨核细胞成熟时，单独使用rIL-6可增加胞浆内血小板糖蛋白（GP IIb IIIa），并且对GP的出现也表现出协同作用IIb IIIa 位于细胞表面。这表明rIL-6对该细胞系的巨核细胞分化也有作用。 3．我们使用体外免疫沉淀法继续对 T-33 细胞的巨核细胞分化进行 c-yes 酪氨酸蛋白激酶 (TPK) 测定。结果，T-33在TPA处理后3至40小时再次显示c-yes TPK活性降低。类似地，K562 在相同处理后 16 至 40 小时显示出下降。另一方面，单核细胞系U937在6至48小时显示增加，而HL-60细胞系显示早期减少和随后在36小时增加。在与 IL-6 孵育的情况下，T-33 的 TPK 活性在 40 小时时增加。与 c-yes 相反，在所有测试的细胞系中，scr 的 TPK 活性通常在 24 至 48 小时内增加。