Mechanism of Megalraryocyhic differentiction-localigation of throwbpoietin receptor and ultrastructure of Megakaryocyte

巨核细胞分化机制-促血小板生成素受体定位及巨核细胞超微结构

• 批准号: 10670195
• 负责人: TANGE Tsuyoshi
• 金额: $ 2.11万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670195/
• 关键词: Megakaryocyte; Differentiation; Thrombopoietin; C-MPl; gold-colloid; Eectron microscope; c-MPL; 免疫電顕; C-mpl; c-mpl; C-Mpl

In our laboratory, immuno-electron microscopyc was applied to demonstrate the location of c-MPL (thrombopoietin receptor) in human megakaryocytes using anti-human c-MPL mouse monoclonal antibody in 1998.For the first two years, fundamental technique of post-embedding gold colloid method 〓sing immuno-electron microscopy was much learned, while in vitro culture of megakaryocytes isolated from bone marrow was continued as already reported to obtain the cell sample for c-MPL study.In conclusion, we failed in demonstrating the location of c-MPL by electro-microscopy, while some ultrastructure of the megakaryocytes were proved to be essential for cell differentiation leading to the production of proplatelets or platelets.

1998年，我们实验室利用抗人c-MPL小鼠单克隆抗体，通过免疫电镜技术证实了c-MPL（血小板生成素受体）在人巨核细胞中的定位。前两年，基本技术是后包埋技术。金胶体法〓免疫电子显微镜学得到了很多知识，同时继续从骨髓中分离出巨核细胞的体外培养，正如已经报道的那样获取细胞样本进行c-MPL研究。 总之，我们未能通过电镜证明c-MPL的位置，而巨核细胞的某些超微结构被证明对于细胞分化导致前血小板或血小板的产生至关重要。

项目成果

Horie, R.,Tange, T. et al.: "Frequent expression of the varient CD3D in human malignant myeloid and lymphoid neoplasms"Am. J. Pathol.. 155(6). 2029-2041 (1999)

Horie, R.、Tange, T. 等人：“变体 CD3D 在人类恶性骨髓和淋巴肿瘤中的频繁表达”Am。

Nishihara, K, Tange, T., et al.: "Tissue immunity, HLA and action of the gravity"J.Oromax.Biomech.. 4(1). 54-57 (1998)

Nishihara, K, Tange, T., et al.：“组织免疫、HLA 和重力作用”J.Oromax.Biomech.. 4(1)。

Horie, R., Tange, T.et al.: "Frequent expression of the variant CD30 in human malignant myeloid and lymphoid neoplasms"Am.J.Pathol.. 155(6). 2029-2041 (1999)

Horie, R.、Tange, T. 等人：“变体 CD30 在人类恶性骨髓和淋巴肿瘤中的频繁表达”Am.J.Pathol.. 155(6)。

Nishihara, K, Tange, T, et. al.: "Tissne immunity , HLA and action of the gravity"J. Oromax. Biomech. 4(1). 54-57 (1998)

西原 K、丹下 T 等。

Horie,R, Tange, T, et al: "Frequent expression of the varient CD30 in human malignant myeloil and lymphoid neoplasms"AM. J. pathol. 155(6). 2029-2041 (1999)

Horie,R, Tange, T 等人：“变体 CD30 在人类恶性骨髓和淋巴肿瘤中的频繁表达”AM。