Natriuretic peptides govern euryhalinity of fishes Analyses using transgenid technology

利钠肽控制鱼类的广盐度 使用转基因技术进行分析

• 批准号: 13304063
• 负责人: TAKEI Yoshio
• 金额: $ 26.12万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13304063/
• 关键词: natriuretic peptides; guanylins; adrenamedullins; molecular evolution; body fluid regulation; cardiovascular action; eel; puffer fish; ウナギ,Anguiila japonica; トラフグ,Takifugu rubripes; トランスジェニック技術; メダカ; 海水適応能; cDNAクローニング

Accumulating data from this laboratory strongly indicate that the natriuretic peptides (NPs) govern euryhalinity of eels ; atrial NP (ANP) promotes seawater adaptation and C-type NP (CNP) promotes freshwater adaptation. In this study, we attempted to determine that ANP is in fact essential for seawater adaptation using transgenic techniques. For this purpose, we use medaka fish that have plural akin species in the same genus that exhibit diverse osmotic adaptability and in which transgenic technique has already been established. Therefore, the production of ANP transgenic seawater medaka that cannot survive in seawater without transferred ANP gene will directly demonstrate the importance of ANP in seawater adaptation. However, ANP cDNA have not been cloned in medaka fish although cloning was successful in several other teleost species. Meanwhile, we could detect four CNPs, named CNP-1 through 4, and B-type NP (BNP) that had been thought as tetrapod-specific NP. Furthermore, we isolated CNP-4 type NPs from most primitive extant vertebrates, lamprey and hagfish. Therefore, we proposed new hypothesis of the molecular evolution of the NP family ; CNP-4 is an ancestral molecule of the NP family and it was diversified in bony fishes in parallel with the evolution of osmoregulatory mechanisms.

该实验室积累的数据强烈表明，钠尿肽（NP）控制着鳗鱼的广盐性；心房NP（ANP）促进海水适应，C型NP（CNP）促进淡水适应。在这项研究中，我们试图利用转基因技术确定 ANP 实际上对于适应海水至关重要。为此，我们使用青鳉鱼，该青鳉鱼在同一属中具有多种类似物种，表现出不同的渗透适应性，并且已经建立了转基因技术。因此，生产没有转移ANP基因就无法在海水中生存的ANP转基因海水青鳉，将直接证明ANP在海水适应中的重要性。然而，尽管在其他几种硬骨鱼物种中克隆成功，但 ANP cDNA 尚未在青鳉鱼中克隆。同时，我们可以检测到四种CNP，分别命名为CNP-1至4，以及被认为是四足动物特异性NP的B型NP（BNP）。此外，我们从大多数原始的现存脊椎动物、七鳃鳗和盲鳗中分离出了 CNP-4 型 NP。因此，我们提出了NP家族分子进化的新假说； CNP-4 是 NP 家族的祖先分子，它在硬骨鱼中随着渗透调节机制的进化而多样化。

项目成果

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Anderson W.G. et al.: "The dipsogenic effect of the rennin-angiotensin system in elasmobranch fish"General and Comparative Endocrinology. 125. 300-307 (2001)

Anderson W.G.等人：“软骨素-血管紧张素系统在软骨鱼类中的作用”普通和比较内分泌学。