Hormonal Control of osmoregulation in euryhaline fis

广盐鱼渗透压调节的激素控制

基本信息

批准号：
06044270
负责人：
TAKEI Yoshio
金额：
--
依托单位：
Ocean Research Institute, University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for Overseas Scientific Survey.
财政年份：
1994
资助国家：
日本
起止时间：
1994 至无数据
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-06044270/
关键词：
Angiotensin Endothelin ANP vasotocin osmoregulation eel rainbow trout flounder

项目摘要

Takei visited Dr.Balment of Manchester University to conduct following experiments using flounder.(1) Papaverine, a vasorelaxant drug, was injected into the flounder, and the time course of drinking rate, plasma angiotensin II and vasotocin levels were measured. The effect of angiotensin II inhibition on drinking rate and plasma vasotocin level after papaverine was also examined.(2) We sequenced eel atrial natriuretic peptide (ANP) in eels for the first time in fishes. Synthetic eel ANP was injected into European eels, and the time course of change in drinking rate and plasma vasotocin level was examined.2. Hyodo also visited Dr.Balment and conducted the following experiments.(1) He found that the expression of vasotocin mRNA decreased after transfer of rainbow trout to seawater, and Dr.Balment found that plasma vasotocin level in the rainbow trout increased after transfer to seawater. Thus, they worked together to conduct similar experiments in the flounder.(2) The time course of change in vasotocin mRNA expression in the paraventricular nucleus of the trout brain was examined by Northern blot analysis.3. Hamano visited Dr.Hazon of University of St.Andrews and conducted the following experiments.(1) She had worked with Dr.Hazon previously for the vasopressor effect of angiotensin II in the dogfish, and found that mechanism of the pressor effect was quite different from that of other animals. She further analyzed the mechanism using an isolated aortic ring of dogfish.(2) She also examined the vasorelaxant activity of homologous C-type natriuretic peptide using isolated aortic ring of dogfish.

Takei访问了曼彻斯特大学的博士，以使用比目鱼进行实验后进行实验。（1）将血管瘤药物的帕帕林注入了比目鱼中，并测量了饮酒率，血浆血管素II和血管增生剂水平的时间过程。还检查了血管紧张素II抑制对爸爸蛋白后饮酒率和血浆血管素水平的影响。（2）我们首次在鱼类中对鳗鱼进行了鳗鱼心房纳二肽（ANP）的测序。将合成的EEL ANP注入欧洲鳗鱼，并检查了饮酒率和血浆血管毒素水平的时间变化。2。 Hyodo还参观了博士并进行了以下实验。（1）他发现，彩虹鳟鱼转移到海水后，血管索素mRNA的表达降低了，博士发现，彩虹鳟鱼在转移到海水后的血浆血管素水平增加。因此，他们共同努力在比目鱼中进行类似的实验。（2）通过Northern blot分析检查了鳟鱼脑室核中血管固醇mRNA表达的变化时间。3。哈马诺访问了圣安德鲁斯大学的哈森博士，并进行了以下实验。（1）她以前曾与哈桑博士合作在狗鱼中为血管紧张素II的加压剂效应，并发现persportor效应的机制是完全不同的来自其他动物。她进一步使用孤立的Dogfish主动脉环进一步分析了该机制。（2）她还检查了使用分离的dogfish主动脉环的同源C型Natriuretic肽的血管曲线活性。