Study on environmental pollution with mutagens in high mortality areas by lung cancer

肺癌高死亡率地区诱变剂环境污染研究

• 批准号: 12680555
• 负责人: WATANABE Tetsushi
• 金额: $ 2.37万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680555/
• 关键词: mutagen; soil; 1,3-dinitropyrene; 1,6-dinitropyrene; 1,8-dinitropyrene; 3-nitrobenzoanthrone; airborne particle; 変異原性物質; ジニトロピレン

1,3-Dinitropyrene (DNP), 1,6-DNP and 1,8-DNP in 30 surface soil samples were quantified by high performance liquid chromatography equipped with a platinum/rhodium catalyst column and a fluorescence detector. DNP isomers were detected in all soil samples examined, and their concentration in soil samples ranged from a few pg/g of soil. 3-Nitrobenzanthrone (NBA) was detected as a major mutagen in a soil sample that showed strong mutagenicity. To clarify the distribution of 3-NBA in the environment, we developed an analytical method of 3-NBA in airborne particles and surface soil. 3-NBA was partially purify by HPLC and quantified by HPLC with a fluorescence detector. Concentrations of 3-NBA in ambient air in the Kinki area were a few pg/m3, and those in soil samples from the Kinki, Tokai and Kanto areas ranged from a few ng to a few hundred ng/g of soil.

采用配备铂/铑催化剂柱和荧光检测器的高效液相色谱法对 30 个表层土壤样品中的 1,3-二硝基芘 (DNP)、1,6-DNP 和 1,8-DNP 进行定量。在所有检查的土壤样品中均检测到 DNP 异构体，其在土壤样品中的浓度范围为几皮克/克土壤。 3-硝基苯并蒽酮 (NBA) 在土壤样品中被检测为主要诱变剂，具有很强的诱变性。为了阐明 3-NBA 在环境中的分布，我们开发了空气颗粒和表土中 3-NBA 的分析方法。 3-NBA 通过 HPLC 进行部分纯化，并通过带有荧光检测器的 HPLC 进行定量。近畿地区环境空气中 3-NBA 浓度为几 pg/m3，近畿、东海和关东地区土壤样品中 3-NBA 浓度为几 ng 至几百 ng/g 土壤。

项目成果

Tetsushi Watanabe et al.: "Mutagenic activity of surface soil and quantification of 1,3-,1,6-and 1,8-dinitropyrene isomers in soil in Japan"Chemical Research Toxicology. 13. 281-286 (2000)

Tetsushi Watanabe 等人：“日本表层土壤的诱变活性以及土壤中 1,3-、1,6-和 1,8-二硝基芘异构体的定量”化学研究毒理学。

Tetsushi Watanabe: "Mutagenic activity of surface soil and quantification of 1,3-, 1,6- and 1,8-dinitropyrene isomers in soil in Japan"Chemical Research in Toxicology. 13. 281-286 (2000)

Tetsushi Watanabe：“日本表层土壤的诱变活性以及土壤中 1,3-、1,6- 和 1,8-二硝基芘异构体的定量”毒理学化学研究。

Tetsushi Watanabe: "Mutagenic activity of Surface soil and quantification of 1,3-,1,6-and 1,8-dinitropyrene isomers in soil in Japan"Chemical Research in Toxicology. 13. 281-286 (2000)

Tetsushi Watanabe：“日本表层土壤的诱变活性和土壤中 1,3-,1,6-和 1,8-二硝基芘异构体的定量”毒理学化学研究。

Tetsushi Watanabe: "Genotoxicity of soil"J. Health Science. 47. 433-438 (2001)

渡边哲志：“土壤的遗传毒性”J.

Tetsushi Watanabe: "Genotoxicity of Soil"J.Health Science. 47. 433-438 (2001)

渡边哲志：“土壤的基因毒性”J.健康科学。