Detection and genotoxicity of nitroarenes in ambient air and surface soil

环境空气和表土中硝基芳烃的检测和遗传毒性

• 批准号: 16590098
• 负责人: WATANABE Tetsushi
• 金额: $ 1.6万
• 依托单位: Kyoto Pharmaceutical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590098/
• 关键词: nitroarene; air; soil; genotoxicity; mutagen

Organic extracts from surface soil samples collected in Osaka and Aichi prefectures showed potent mutagenicity in S.typhimurium TA98 in the absence of S9 mix, inducing 1,240 - 233,000 revertants/mg of organic extract. Each soil extract was separated by chromatography using a Sephadex LH-20 column and a silica gel column. The fractions with potent mutagenicity were subsequently separated into five fractions, Fr. 1 - Fr. 5, using an Ultra pack ODS column. The contribution ratios of these five fractions to the total mutagenicity of each soil extract were similar ; Fr. 1 : 45% ; Fr. 2 : 15% ; Fr. 3 : 25% ; Fr. 4 : 5 ; Fr. 5 : 10%. These five fractions were further separated by HPLC. In consequence, it was found that mutagenic potencies of Fr. 1 and Fr. 3 were attributed to dinitropyrene (DNP) and 3,6-dinitrobenzo[e]pyrene (DNBeP), respectively. Major mutagens in Fr. 2, Fr. 4, and Fr. 5 were isolated from each soil extract, and these mutagens were expected to be nitroarenes on the basis of their mass spectra. Retention times of these mutagens in HPLC were not consistent with those of nitrosrenes, which had been detected in the environment. 3,6-DNBeP showed extremely potent mutagenicity in TA98 without S9 mix, inducing 285,000 revertants/nmol. This potency was comparable to that of 1,8-DNP, which is the strongest mutagen reported to date. Because 3,6-DNBeP showed higher mutagenicity in YG1024 than TA98, 3,6-DNBeP was thought to be activated by O-acetyltranferase for mutagenicity.

在没有S9混合物的情况下，从大阪收集的表面土壤样品和AICHI县收集的有机提取物显示出有效的诱变性，诱导了1,240-233,000个恢复/毫克的有机提取物。使用Sephadex LH-20色谱柱和二氧化硅凝胶柱通过色谱分离每个土壤提取物。随后将有效诱变的分数分为五个分数。 1 -Fr。 5，使用Ultra Pack ODS列。这五个部分对每个土壤提取物的总诱变的贡献比相似。神父1：45％；神父2：15％；神父3：25％；神父4：5;神父5：10％。这五个部分通过HPLC进一步分离。因此，发现神父的诱变效力。 1和神父3分别归因于二硝基林（DNP）和3,6-二硝基苯并[E] pyrene（DNBEP）。神父的主要诱变剂。 2，神父4和神父从每种土壤提取物中分离出5，预计这些诱变剂将根据其质谱为硝化。这些诱变剂在HPLC中的保留时间与在环境中检测到的硝酸盐的保留时间不一致。 3,6-DNBEP在没有S9混合物的TA98中表现出极有效的诱变，诱导285,000个恢复体/Nmol。这种效力与1,8-DNP相当，这是迄今为止报告的最强诱变剂。由于3,6-DNBEP在YG1024中显示出高于TA98的诱变症，因此被认为3,6-DNBEP被O-乙酰基转促酶激活了诱变酶。

项目成果

Detection of a Novel Mutagen, 3,6-Dinitrobenzo[e]pyrene, as a Major Contaminant in Surface Soil in Osaka and Aichi Prefectures, Japan

在日本大阪和爱知县表层土壤中检测到一种新型诱变剂 3,6-二硝基苯并[e]芘作为主要污染物

• 发表时间: 2005
• 期刊: Chemical Research in Toxicology 18
• 作者: K.Sugihara;S.Kitamura;T.Yamada;T.Okayama;S.Ohta;Tetsushi Watanabe
• 通讯作者: Tetsushi Watanabe

Mutagenicity of Surface Soils in Urban Areas of Aichi Prefecture, Japan, and Bangkok, Thailand

日本爱知县和泰国曼谷城市地区表层土壤的致突变性

• 发表时间: 2005
• 期刊: Journal of Health Science 51(6)
• 作者: Hashimoto Y;Kaneko Y;Tsukamoto E;Frankowski H;Kouyama K;Kita Y;Niikura T;Aiso S;Bredesen DE;Matsuoka M;Nishimoto I;Kitamura et al.分担(R.C.Gupta編集);Tetsushi Watanabe
• 通讯作者: Tetsushi Watanabe