Histological tissue response and synovial fluid analysis after arthroscopic surgery with electrocautery in TMJ

颞下颌关节镜电灼手术后的组织学组织反应和滑液分析

基本信息

批准号：
12671977
负责人：
KAKUDO Kenji
金额：
$ 2.05万
依托单位：
OSAKA DENTAL UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671977/
关键词：
TMJ ARTHROSCOPY ELECTROCAUTERY WOUND HEALING SHEEP SYNOVIAL FLUID

项目摘要

Aims : To evaluate the tissue response and synovial fluid analysis after arthroscopic lysis with electrocautery in the temporomandibular joint (TMJ) of sheep histologically, radiologically and biochemicallyMethod : Thirteen adult merino sheep, weighting about 60 kg, were used. The instruments used in this study were a newly developed artroscope with a channel (1.1mm in diameter) for TMJ surgery (Int. J. Oral Maxillofac. Surg.26S : 159, 1997), Electrocautery was inserted into the inner channel and the tip placed directly against the synovial membrane of the posterior pouch and the surface was injured from medial to lateral walls by electrocautery with a power of 125 watts. The tip is then placed against the articular surface of the tuber which was damaged from medial to lateral part with electrocautery until the underlining bone was exposed. At the 2nd, 4th, 8th, 12th week after the initial operation, the sheep were sacrificed by an anesthetic overdose. The specimens were decalified and sagitally sectioned from lateral to medial for histological study. Synovial fluid was collected at same time. Casein zymography was performed to detect the presence of MMP-3 in synovial fluidResults : At the 4th week after the initial operation, all discs were perforated. At the 12th week after the initial operation, the condylar articular surface was partly denuded in some areas, and part of the surface showed fibrous repair, and the disc was displaced ot the anterior portion. These canges were radiographically and histologically similar to adovanced osteoarthritis. Pro MMP-3 was detected in all experimental groupsConclusion : It was suggested that an arthroscopic lysis with electrocautery agaist the synovial membrance of the posterior pouch was induced the osteoarthritic change of sheep TMJ

目的：从组织学、放射学和生化方面评估羊颞下颌关节 (TMJ) 电灼关节镜松解术后的组织反应和滑液分析方法：使用 13 只成年美利奴羊，体重约 60 kg。本研究使用的器械是新开发的带有通道（直径1.1mm）的关节镜，用于TMJ手术（Int. J. Oral Maxillofac. Surg.26S : 159, 1997），将电灼器插入内部通道和尖端将其直接贴在后囊滑膜上，用125瓦功率的电灼器从内侧到外侧损伤表面。然后将尖端放置在结节的关节表面上，通过电灼从内侧到外侧部分损坏，直到暴露下衬骨。初次手术后第2、4、8、12周，因麻醉过量处死羊。将标本脱钙并从外侧到内侧进行矢状切片以进行组织学研究。同时收集滑液。进行酪蛋白酶谱检测滑液中 MMP-3 的存在。结果：初次手术后第 4 周，所有椎间盘均穿孔。初次手术后第12周，髁突关节面部分区域裸露，部分表面出现纤维修复，椎间盘向前部移位。这些病变在放射学和组织学上与晚期骨关节炎相似。所有实验组均检测到Pro MMP-3结论：提示关节镜下电灼松解后囊滑膜可引起绵羊颞下颌骨关节炎改变。