Development of the Artificial Nerve Using Laminin Peptides and rhMDP

使用层粘连蛋白肽和 rhMDP 开发人工神经

基本信息

批准号：
12671399
负责人：
ITOH Soichiro
金额：
$ 2.43万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

项目摘要

We have developed tendon chitosan tubes having the property to covalently bind peptides, and the effectiveness of laminin peptides coupled to these tubular wall on nerve regeneration was examined in vivo. Bridge graft implantation (15mm) into the sciatic nerve of SD rats was carried out using chitosan tubes having a triangular cross section containing either covalently bound intact laminin, the laminin peptides CDPGYIGSR or CSRARKQAASIKVAVSAD, or being non-treated (N=20 in each group). As a control, isografting (N=5) was carried out. Three rats in each experimental group were sacrificed for histology observations after respectively 1, 2, 4, 6 and 8 weeks. The total area of regenerating tissue in the tube and the length of the area where regenerating tissue attached to the inner surface of the tube were measured. In five rats from each experimental and control group, the latency quotient between the implanted and non-treated site was determined 12 weeks after implantation. Furthermore, the percentage of the myelinated axon area was measured at a 10 mm distance of the distal anastomosed site. Histological findings suggest that the immobilized laminin confirmed by immunostain as long as 12 weeks post operatively, as well as laminin oligopeptides may effectively assist nerve tissue extension. According to statistical analysis of the percentage neural tissue found in relation to evoked action potentials, the sequential treatments with YIGSR first followed by IKVAV matched the effectiveness of intact laminin in order to enhance nerve regeneration. However, when compared with isografting, the enhancement of regenerated axon growth was less sufficient.

我们已经开发了具有共价肽的肌腱壳管，并在体内检查了与这些管状壁偶联的层粘连蛋白肽的有效性。使用具有三角形横截面的壳聚糖管进行SD大鼠坐骨神经的桥接移植物（15mm），该管子包含共价结合的完整层粘连蛋白，层粘连蛋白肽CDPGYIGSR或CSRARKQAASIKIKAVSAD，或者是未磨损的（N = 20）。作为对照，执行Isografting（n = 5）。在分别为1、2、4、6和8周之后，为每个实验组中的三只大鼠进行组织学观察。测量了试管中再生组织的总面积和附着在管子内表面的区域的长度。在每个实验组和对照组的五只大鼠中，植入后12周确定植入和未处理位点之间的潜伏商。此外，在远端吻合位点的10 mm距离处测量髓鞘轴突区域的百分比。组织学发现表明，在手术后12周以及层粘连蛋白寡肽可以有效地有效地帮助神经组织延伸。根据对与诱发作用电位相关的神经组织百分比的统计分析，首先使用YIGSR的顺序处理后，IKVAV与完整层粘连蛋白的有效性相匹配，以增强神经再生。但是，与Isografting相比，再生轴突生长的增强较少。