A STUDY ON DEVELOPMENT OF THE ARTIFICIAL NERVE AND ESTABLISHMENT OF THE NEW METHOD TO ASSESS THE PERIPHERAL NERVE REGENERATION.

人工神经发育的研究及周围神经再生评估新方法的建立。

• 批准号: 10671348
• 负责人: ITOH Soichiro
• 金额: $ 2.11万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671348/
• 关键词: TCS-CMAP; MDP77; Neurocrescin; artificial nerve; scaffold for nerve regeneration; type I collagen; collagen membrane for GTR; collagen fiber; 経頭蓋刺激末梢筋誘発電位測定; type Icollagen; GTR用collagen膜; 神経架橋用scaffold; 神経再生; collagen tube; F460; 運動神経再生

(1) As a new method to evaluate the nerve regeneration of the rat sciatic nerve, transcranial stimulation compound muscle action potential (TCS-CMAP) was used together with the sciatic function index (SFI), and recovery of motor function of the rats' lower legs could be evaluated accurately.(2) Using transplantation of the silicone tube into 15mm-gap of the rat sciatic nerve, the efficacy of muscle-derived neurite promoting factor (mNPF) on motor nerve regeneration was histologically evaluated. It was suggested that Muscle Derived Protein 77 (MDP77) promotes sprouting of the regenerating axons and Neurocrescin has combined action to promote sprouting and maturation of the regenerating axons by dose dependent manner.(3) Tubes made of a beta-tricalcium phosphate/polylactic acid composite (TCP/CPLA) and silicone tubes, packed with bioabsorbable fibers, either atelocollagen fibers or fibers made of copolymerized poly-L-lactide (CPLA : copolymer of poly-L-lactide and poly(ethylenesebacate-co-hexamethylenesebacate)), were each grafted to bridge a gap of 15 mm in a rat sciatic nerve. The TCP/CPLA tubes remained present without being absorbed and were collapsed 8 weeks after surgery. Although minifascicles were formed between the CPLA fibers, these fibers remained present without disintegrating and occupied a large space. Many minifascicles were observed between the collagen fibers, and immunohistological analysis revealed that Schwann cells had infiltrated the region consisting of degenerated collagen fibers. The results of grafting using tubes made from a collagen sheet or a guided tissue regeneration (GTR) membrane with inserted collagen fibers or films were evaluated using the same model. Both the collagen tube and the GTR tube were available for tubulization, and these tubes packed with collagen fibers were effective to provide the scaffold for regenerating axons and Schwann cells.

（1）作为评估大鼠坐骨神经再生的新方法，将经颅刺激复合肌肉动作电位（TCS-CMAP）与坐骨神经功能指数（SFI）一起使用，并且可以准确评估大鼠下腿运动功能的运动功能，并可以准确评估（2）使用硅胶含量为15mmm-gap的capial fef。对运动神经再生的肌肉衍生的神经突促进因子（MNPF）进行了组织学评估。有人提出，肌肉衍生的蛋白77（MDP77）促进了再生轴突和神经霉素的发芽，其作用结合了作用，以促进通过剂量依赖的方式促进再生轴突的发芽和成熟。可生物吸附的纤维，即与共聚物聚合纤维或共聚物聚合聚合物纤维（CPLA：聚-l-丙二醇的共聚物：聚-l-甲酸的共聚物和聚（乙烯基 - 乙酸 - 甲基己酯）的共聚物），分别嫁接到15 mm的rap sciat sciat sciat sciat sciat sciat sciat sciatic nerver中，分别嫁接为桥梁。 TCP/CPLA管仍然存在而没有被吸收，并在手术后8周倒塌。尽管CPLA纤维之间形成了微型囊泡，但这些纤维仍然存在而没有分解和占据很大的空间。在胶原蛋白纤维之间观察到许多微型囊泡，免疫组织学分析表明，Schwann细胞渗透了由退化的胶原纤维组成的区域。使用相同的模型评估了使用胶原板或带导的组织再生（GTR）膜制成的试管（GTR）膜的结果。胶原管和GTR管均可用于管状，这些带有胶原蛋白纤维的管有效地为再生轴突和雪旺氏细胞提供了支架。

项目成果

若林良明: "運動神経を標的とする再生医療の役割"バイオインダストリー. 17(2). 34-41 (2000)

Yoshiaki Wakabayashi：“针对运动神经的再生医学的作用”Bioindustry 17(2) (2000)。

鮫島弘武: "経頭蓋電気刺激-誘発筋電図導出法を用いたラット末梢神経運動機能評価法"脊髄電気診断学. 21(1). 36-39 (2000)

Hirotake Samejima：“使用经颅电刺激诱发肌电图推导法评估大鼠周围神经运动功能”《脊髓电诊断》21（1）。

S. Itoh, H. Samejima, K. Shinomiya, A. Awaya: "The effect of neurotrophic pyrimidine heterocyclic compounds, MS-818 and MS-430, on the regeneration of injured peripheral nerves."Restorative Neurology and Neuroscience. 14. 265-273 (1999)

S. Itoh、H. Samejima、K. Shinomiya、A. Awaya：“神经营养性嘧啶杂环化合物 MS-818 和 MS-430 对受损周围神经再生的影响。”恢复神经病学和神经科学。

Y. Wakabayashi, K. Shinomiya, S. Itoh: "A role of regenerative medicine focused on motor neuron."Bioindustry. 17(2). 34-41 (2000)

Y. Wakabayashi、K. Shinomiya、S. Itoh：“再生医学的作用集中在运动神经元。”生物工业。