Effects of Electrical Polarization of Hydroxyapatite ceramics on Bone Conduction

羟基磷灰石陶瓷的电极化对骨传导的影响

基本信息

批准号：
17500302
负责人：
ITOH Soichiro
金额：
$ 2.24万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

Experiment 1.Large surface charges can be induced on hydroxyapatite (HAp) ceramics by proton transport polarization, but this does not affect β-tricalcium phosphate (β-TCP) because of its low polarizability. We wished to examine differences in osteogenic cell activity and new bone growth between positively or negatively surface charged HAp and HAp/TCP plates using a calvarial bone defect model. In the first group of rats, test pieces were placed with their positively charged surfaces face down on the dura mater. In a second group, test pieces were placed with their negatively charged surfaces face down on the dura mater. A third group received non-charged test pieces. Histological examination, including enzymatic staining for osteoblasts and osteoclasts, was carried out. While no bone formation was observed at the pericranium, direct bone formation on the cranial bone debris and new bone growth expanded from the margins of the sites of injury to bridge across both the positively and ne … More gatively charged surfaces of HAp and HAp/TCP plates occurred. Electrical polarization of implanted plates, including positive charge, led to enhanced osteoblast activity, though decreased osteoclast activity was seen on the positively charged plate surface. Thus, polarization of HAp ceramics may modulate new bone formation and resorption.Experiment 2.Hydroxyapatite ceramics are used as implants to repair damaged/removed bone, and negative or positive electrical polarization enhances osteoblast and decreases osteoclast activity, respectively, in vivo. We compared the ability of electrically polarized and non-polarized hydroxyapatite with interconnected pores (IPHA) implants to promote bone growth. Polarized or non-treated IPHAs were implanted into the right or left femoral condyle of rabbits (N=10 in each group), and we performed histological examination, including enzymatic staining for osteoblasts and osteoclasts, three and six weeks after implantation. We observed improved bone ingrowth and increased osteoblast activity in polarized implants with complete bone penetration into polarized implants occurring as early as three weeks after surgery. In contrast, non-polarized implants were not fully ossified at six weeks after surgery. Furthermore, positively charged implant regions had decreased osteoclast activity compared to negatively charged or uncharged regions. We propose two different models to explain these observations. Less

实验1.可以通过质子转运极化在羟基（HAP）陶瓷上诱导LARGE表面电荷，但由于其极化性较低，因此不会影响β-三氯磷酸盐（β-TCP）。我们希望使用钙质骨缺损模型检查成骨细胞活性的差异和带负电荷的HAP和HAP/TCP平板之间的新骨生长。在第一组大鼠中，将测试件放在硬脑膜上的带正电荷的表面。在第二组中，将测试件放置在硬脑膜上的带负电荷的表面。第三组收到了非收费测试作品。进行了组织学检查，包括成骨细胞和破骨细胞的酶促染色。虽然在腹膜上未观察到骨形成，但颅骨碎屑上的直接骨形成，新的骨骼生长从损伤部位的边缘扩展到跨越正面和NE的桥梁……HAP和HAP/TCP平板的更积极的电荷表面。植入板的电偏振（包括正电荷）导致成骨细胞活性增强，尽管在带正电荷的板表面上看到了破骨细胞活性的减少。这是HAP陶瓷的极化可能会调节新的骨形成和分辨率。2。羟基磷灰石陶瓷被用作即兴修复受损/去除的骨骼，负或阳性的极性分别增强了成骨细胞并降低骨细胞活性。我们比较了电化和非偏振羟基磷灰石与互连孔（IPHA）促进骨骼生长的能力。极化或未处理的IPHAS被植入兔子的右或左股骨con（每组n = 10），我们进行了组织学检查，包括植入后三和六周的成骨细胞和整骨细胞的酶促染色。我们观察到在术后三周后，完全骨穿透到极化不完美的情况下，骨骼侵蚀的改善和成骨细胞的活性增加。相比之下，在手术后六周后，未完全骨化了非极化的不完美。此外，与带负电荷或未充电的区域相比，带正电的不完美区域的破坏性区域恶化。我们提出了两个不同的模型来解释这些观察结果。较少的