Anti-Aging Effects of Natural Antioxidants on Senescence Accelerated Mouse (SAM)

天然抗氧化剂对加速衰老小鼠 (SAM) 的抗衰老作用

基本信息

批准号：
63580072
负责人：
YAMASHITA Kanae
金额：
$ 1.54万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1990
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63580072/
关键词：
SAM sesame sesaminol catechin Vitamin E peroxidized lipids anti-aging effects

项目摘要

To investigate the effects of antioxidants in foods on the aging of biological function, senescence accelerated mice (SAM) were used throughout this study as a test animal, in addition to Wistar rats. Sesame seed products, sesaminol isolated from sesame oil and catechin isolated from green tea were used as antioxidants and the following results were obtained.1) By long term feeding with the diets containing high levels of crushed sesame seeds, the peroxidation of lipids in the liver and increases of lipofuscin in the testis were effectively depressed in comparison with the control group without crushed sesame seeds.2) The addition of crushed sesame seeds to the diets suppressed the grading score for evaluation of the degree of senecence of SAM, especially the score for glossiness and coarseness of skin hair.3) Peroxidized lipids in the liver and plasma, and hemolysis and pyruvate kinase activity in the plasma were markedly increased in rats fed a tocopherol deficient diet. The addition of sesame products (crushed sesame seeds and oils) which contained -tocopherol exclusively, lowered these characteristics to the same level as the diets containing -tocopherol. However, the addition of -tocopherol to the tocopherol deficient diet depressed the characteristics less than the diet containing -tocopherol.4) The increase of peroxidized lipids in the liver of SAM fed with tocopherol deicient diets was depressed by the addition of sesaminol (0.1%), a potent antioxidative lignan in sesame oil. The dosage of carbon tetra chloride solution to rats also induced lipid peroxidation in its liver, but it was suppressed to an ordinary level by the addition of sesaminol to the solution.5) On the same experiments using tocopherol deficient diets, a slight depression of lipid peroxidation in liver, kidney and plasma was observed by the addition of catechin and its analogues from green tea to the diets.

为了研究食物中的抗氧化剂对生物功能衰老的影响，除 Wistar 大鼠外，整个研究中还使用衰老加速小鼠 (SAM) 作为测试动物。以芝麻制品、芝麻油中分离的芝麻素和绿茶中分离的儿茶素为抗氧化剂，得到以下结果。 1）长期饲喂含有高含量碎芝麻的日粮，肝脏中脂质过氧化与未添加碎芝麻的对照组相比，睾丸中脂褐质的增加得到有效抑制。2）在日粮中添加碎芝麻可抑制评分用于评价SAM的衰老程度，特别是皮毛光泽度和粗糙度的评分。3)缺乏生育酚饮食的大鼠肝脏和血浆中的过氧化脂质以及血浆中的溶血和丙酮酸激酶活性显着增加。添加仅含有α-生育酚的芝麻产品（碎芝麻和芝麻油）可将这些特性降低至与含有α-生育酚的饮食相同的水平。然而，在缺乏生育酚的日粮中添加α-生育酚对特性的抑制作用小于含有α-生育酚的日粮。4)添加芝麻素酚(0.1%)抑制了饲喂缺乏生育酚日粮的SAM肝脏中过氧化脂质的增加。），芝麻油中的一种有效的抗氧化木脂素。给予大鼠四氯化碳溶液的剂量也会诱导其肝脏中的脂质过氧化，但通过向溶液中添加芝麻素酚将其抑制至正常水平。 5) 在使用缺乏生育酚的饮食的相同实验中，脂质过氧化略有降低通过在饮食中添加绿茶中的儿茶素及其类似物，观察到肝脏、肾脏和血浆中的过氧化作用。