Protective effects of sesame lignans on senescence accelerate brain in rats and mice

芝麻木酚素对大鼠和小鼠大脑衰老的保护作用

基本信息

批准号：
12680143
负责人：
YAMASHITA Kanae
金额：
$ 2.37万
依托单位：
Sugiyama Jogakuen University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12680143/
关键词：
sesame seed sesame lignans α-tocopherol senescence of brain rats SAMP8 TBARS learning test

项目摘要

Recent studies have shown that oxidative stress is important in the pathogenesis of Alzheimer disease and high doses of vitamin E to Alzheimer disease patients improved the disease state. β-Amyloid (Aβ), which is found abundantly in the brains of Alzheimer disease patients, is cytotoxic to neurons and has a central role in the pathogenesis of the disease. There was a paper to report protective effects of α-tocopherol (α-Toc) on Aβ(1-42)-induced learing and memory deficits in rats. Our previous studies showed that sesame seed and its lignans elevated α-Toc concentrations in brain as well as plasma and liver. In this study, we investigated whether the protective effects of sesame seed on senescence accelerated brain were observed in Aβ infused rats and senescence accelerated mouse (SAM)P8. In Experiment 1, we examined α-Toc concentrations in cerebrum, cerebellum, brain stem and hippocampus in brain and TEARS in brain in rats fed diets containing 500 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 2, learning and memory in radial maze task were examined in rats infused Aβ and fed diets 50 mg/kg α-Toc alone and 50 mg/kg α-Toc with sesame seed. In Experiment 3, passive avoidance task was examined in SAMP8 mice fed the same diets in Exp.2. We obtained the results that sesame seed group showed higher concentrations of α-Toc in all parts of brain examined compared to 500 mg/kg α-Toc group and lower concentration of TEARS in brain, however we could not observe that sesame seed improved learning and memory on senescence accelerated brain in rats and mice.

最近的研究表明，氧化应激在阿尔茨海默病的发病机制中很重要，给阿尔茨海默病患者服用高剂量的维生素E可以改善阿尔茨海默病患者大脑中大量存在的β-淀粉样蛋白（Aβ）。对神经元具有细胞毒性，并在该疾病的发病机制中发挥核心作用有一篇论文报道了 α-生育酚 (α-Toc) 对 Aβ(1-42) 诱导的租赁和记忆缺陷的保护作用。我们之前的研究表明，芝麻及其木脂素可提高大脑以及血浆和肝脏中的 α-Toc 浓度。在这项研究中，我们研究了芝麻对注入 Aβ 的大鼠和加速大脑衰老的保护作用。衰老加速小鼠 (SAM)P8 在实验 1 中，我们检查了喂食饮食的大鼠大脑、小脑、脑干和海马中的 α-Toc 浓度以及大脑中泪液的浓度。单独含有 500 mg/kg α-Toc 和 50 mg/kg α-Toc 与芝麻在实验 2 中，检查了注射 Aβ 并喂食单独 50 mg/kg α-Toc 和 50 mg/kg α-Toc 的大鼠在径向迷宫任务中的学习和记忆能力。 50 mg/kg α-Toc 和芝麻在实验 3 中，对饲喂与实验 2 相同饮食的 SAMP8 小鼠进行被动回避任务检查。与 500 mg/kg α-Toc 组相比，芝麻组在所检查的大脑所有部位中显示出更高的 α-Toc 浓度，并且大脑中 TEARS 的浓度更低，但我们无法观察到芝麻改善了加速大脑衰老的学习和记忆在大鼠和小鼠中。