Development of Active Transport and Ion-Channel Sensors

主动传输和离子通道传感器的开发

• 批准号: 63430008
• 负责人: UMEZAWA Yoshio
• 金额: $ 22.78万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (A)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1990
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63430008/
• 关键词: Ion-channel; Active transport; Chemical sensor; Biosensor; Langmuir-Blodgett Membrane; Bilayer lipid membrane; 脂質分子膜; LB膜; 情報変換@増幅; 電気化学センサ-; 化学センサー; 生体類似センサー; 分子認識; 情報変換; 増幅

Three different types of sensors were constructed. (1) A series of ion-channel type sensors responsive to organic and inorganic anions were constructed utilizing Langmuir-Blodgett membranes containing lipophilic derivatives of macrocyclic polyamine and cyclodextrin polyamine, capable of binding with anionic guests in multiprotonated forms. The sensor showed selective response (modulation of membrane permeability to a number of guest anions including adenosine nucleotides, dicarboxylates and inorganic anions. (2) A biological ion-channel sensor was fabricated by incorporating glutamate receptorion-channel protein, isolated from synaptic membrane of rat brain, into a planar bilayer lipid membrane (BLM) . A signal amplification of 2.1x10^5 was obtained based on the glutamate-triggered single channel current. (3) A Na^+/D-glucose co-transporter protein, isolated from intestinal brush border membrane of guinea pigs, was incorporated into a bilayer membrane to construct an active transport type BLM sensor for D-glucose. The response of the sensor was measured as a conductance change arising from co-transported Na^+ ion flux under a constant applied potential. The response of the sensor was induced by D-glucose above 10^<-9> M, but not by the corresponding L-isomer, D-fructose and D-mannose.

构建了三种不同类型的传感器。 (1)利用含有大环多胺和环糊精多胺亲脂衍生物的Langmuir-Blodgett膜构建了一系列对有机和无机阴离子敏感的离子通道型传感器，能够与多质子化形式的阴离子客体结合。该传感器表现出选择性响应（调节膜对多种客体阴离子的通透性，包括腺苷核苷酸、二羧酸盐和无机阴离子。（2）通过掺入从大鼠突触膜分离的谷氨酸受体离子通道蛋白来制造生物离子通道传感器基于谷氨酸触发的单通道电流，获得了 2.1x10^5 的信号放大 (3)。从豚鼠肠刷状缘膜中分离出Na+/D-葡萄糖共转运蛋白，将其整合到双层膜中以构建D-葡萄糖的主动转运型BLM传感器。传感器的响应测量为：在恒定施加电势下由共转运的 Na^+ 离子通量引起的电导变化。传感器的响应是由高于 10^-9M 的 D-葡萄糖引起的，但不是由相应的 L-异构体引起的。 D-果糖和D-甘露糖。

项目成果

M. SUGAWARA, M. KATAOKA, K. ODASHIMA, AND Y. UMEZAWA: "Biomimetic ion-channel sensors based on host-guest molecular recognition in langmuir-blodgett membrane assemblies" Thin solid films. 180. 129-133 (1989)

M. SUGAWARA、M. KATAOKA、K. ODASHIMA 和 Y. UMEZAWA：“基于朗缪尔-布洛杰特膜组件中主客体分子识别的仿生离子通道传感器”固体薄膜。

M. KATAOKA, M. TAKINAMI, H. ABE, Y. UMEZAWA: "Amplified determination of metal ions with liposomes incorporating hydrophobic chelating agents" Bunseki kagaku. 39. 789-792 (1990)

M. KATAOKA、M. TAKINAMI、H. ABE、Y. UMEZAWA：“用掺有疏水螯合剂的脂质体放大金属离子测定”Bunseki kagaku。

菅原 正雄: "膜を用いるイオン・分子認識の新しいアプロ-チ" 膜. 15. 112-120 (1990)

Masao Sukawara：“使用膜进行离子/分子识别的新方法”膜 15. 112-120 (1990)。

小田嶋 和徳(共著): "機能性大環状化合物の分析化学への応用" アイピ-シ-, 78 (1990)

Kazunori Odashima（合著者）：“功能性大环化合物在分析化学中的应用”IPPC，78（1990）

Y.Umezawa: "IonーSelective Electrodes,5" Akademiai Kiado and Pergamon Press, 675 (1989)

Y. Umezawa：“离子选择电极，5”Akademiai Kiado 和 Pergamon Press，675 (1989)