Identification of chromosome and caryotype analysis in citrus by GISH method and in situ PCR method.

GISH法和原位PCR法鉴定柑橘染色体及核型分析

基本信息

批准号：
12660029
负责人：
KITAJIMA Akira
金额：
$ 2.37万
依托单位：
Kochi University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12660029/
关键词：
citrus chromosome caryotype Giemsa CMA GISH chromosome pairing set polyploid CMA 染色体識別染色体構成染色体対合様式

项目摘要

Although citrus chromosomes, 2n=18, are classified to 5 types (A〜E) by the banding patterns of CMA staining, it is difficult to identify a chromosome in the same type. In this research, CMA staining and GISH method were performed for an identification of chromosome in the same type, and a caryotype analysis of hybrid seedlings from 'Tosa-Buntan' x 'Suisho-Buntan' pummelo was performed to clarify the chromosome paring set of 'Tosa-Buntan'1. Thirteen kinds of chromosome composition were manifested by 38 diploid seedlings from 'Tosa-Buntan' x 'Suisho-Buntan'. The caryotype analysis of the diploid seedlings indicated that the chromosome parting set of 'Tosa-Buntan' would by AB+CC+CC+CD+DE+EE+EE+EE+EE or AB+CC+CC+DD+CE+EE+EE+EE+EE. The caryotype analysis of the tri-and tetraploid seedlings showed that the triploid and tetraploid seedlings from diploid crossing also originated from an unreduced male gamete as well as from an unreduced male gamete.2. By using the relative size of CMA(+) regions as indices, type-C chromosomes were able to classify into two groups in 'Tanikawa-Buntan' and sour orange, and type-D chromosomes were able to classify into three groups in 'Yoshida-Ponkan' and sour orange, and into two groups in 'Mukaku-Kishu'.3. In GISH method, 9 chromosomes of 'Tosa-Buntan' in type-A to E were able to classify into 8 groups depended on coloration and size of the signals by using satsuma mandarin DNA as a probe. Nine chromosomes of 'Suisho-Buntan' in type-A to E were able to classify into 7 groups depended on coloration and size of the signals by using 'Dancan' grapefruit DNA as a probe.4. The GISH for 'Tosa-Buntan' chromosome using several kinds of probe DNA showed that 'Tosa-Buntan' is very closed to 'Suisho-Buntan', relative close to citron and relative far from mandarin and orange. The reciprocal GISH between 'Tosa-Buntan' and navel orange suggested that the same type chromosome between these species may not be homoeologous chromosome.

尽管在这项研究中，CMA染色和Gishod在同一类型的染色体中进行了鉴定，并进行了CMA染色和Gishod，并进行了“ Tosa-Buntan” X'Suisho-Vuntan的杂种型染色体分析“ Tosa-vuntan'1”的染色体折叠术。 CC+CD+DE ++ EE+EE+EE+EE+EE或AB+CC+DD+EE+EE+EE+EE。以及使用CMA（+）区域的相对大小，可以分为“ Tanikawa-Buntan”和Sourange，以及？在“ mukaku ethod”中，在A型A型tosa-buntan'染色体中分成几组可以分为8组，依赖着色和satsuma cansarin dna dna尽可能多地分类。 -buntan'在A型中分为7组，取决于使用葡萄柚DNA作为探针的颜色和大小。4。 -buntan'，相对靠近Citron，远离普通话和橙色。