Is arrested embryo development of seedless Kishu mandarin related to genomic imprinting gene?

无核纪州柑胚胎发育停滞与基因组印记基因有关吗？

• 批准号: 24658029
• 负责人: KITAJIMA Akira
• 金额: $ 2.58万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24658029/
• 关键词: 果樹; カンキツ; 無核性; 胚乳; ゲノムインプリンティング

It is reported that MEA, DME, MET1, FIS2, FWA, PHE, SSRP1 are genomic imprinting genes in Arabidopsis. Citrus homologue genes of these genomic imprinting genes were CLF, SWN, DME, ROS, MET1, FIS2, FWA, FWA2, PHEa, PHEb and SSRP. Genomic DNA and cDNA of Seedless Kishu, Seedy Kishu mandarin and Otachibana pummelo were used for PCR by specific primer of these citrus homologue genes. The amplified products were sequenced. The SNPs were detected in 9 genes of genomic DNA and 8 genes of cDNA. To investigate the genomic imprinting gene expressions in endosperm or embryo crossed by Kishu and Otachibana, different homozygous SNP sequence between Kishu and Otachibana imprinting gene is necessary. These SNP polymorphisms are detected in FWA and PHEb.The expression of FWA in young seeds resulted from Otachibana X Seedless Kishu was investigated, and only 'Seedless Kishu' FWA was expressed. This result indicated that citrus FWA would be genomic imprinting gene.

据报道，MEA，DME，MET1，FIS2，FWA，PHE，SSRP1是拟南芥中的基因组烙印基因。这些基因组烙印基因的柑橘类同源基因是CLF，SWN，DME，ROS，MET1，FIS2，FWA，FWA，FWA2，PHEA，PHEA，PHEB和SSRP。这些柑橘类同源物基因的特定底漆使用了无种子kishu，Seedy Kishu普通话和Otachibana Pummelo的基因组DNA和cDNA。测序放大的产品。在9个基因组DNA和8个cDNA基因中检测到SNP。为了研究胚乳和otachibana交叉的胚乳或胚胎中的基因组印记表达，需要不同的kishu和Otachibana印迹基因之间的不同纯合SNP序列。在FWA和PHEB中检测到这些SNP多态性。研究了由Otachibana X无种子Kishu产生的FWA的表达，并且仅表达了“无籽Kishu” FWA。该结果表明柑橘FWA将是基因组印记基因。