Fundamental study for citrus chromosome mapping

柑橘染色体定位的基础研究

• 批准号: 08660034
• 负责人: KITAJIMA Akira
• 金额: $ 1.15万
• 依托单位: Kochi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 1997
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08660034/
• 关键词: citrus; chromosome; karyotype snslysis; fluorescent stain

Major purpose of this study is to develop new chromosome preparation method for citrus karyotype analysis that is important of gene chromosome mapping, and the second is to try citrus karyotype analysis using this new method and to develop useful chromosome banding method for identification of citrus chromosome.1.Optimum enzymatic treatment was 120 min.incubation of 2% cellulase + 1% pectolyase or 90 min.incubation of 3% cellurase + 2% pectolyase for root tip of in vitro cultured plantlet, and 90min.incubation of 3% cellurase + 1% pectolyase for young leaf tip from orchard tree. The chromosome length of the longest one in each chromosome setreached 4-10mum that was over 2-5 times of the longest chromosome length which had been reported.2.The chromosome numbers of 2n=18 in Poncirus and orange, 2n=20-21 in 'Tosa-Buntan' and 'Suisho-Buntan', 2n=19 in satsuma mandarin, 2n=20 in Fortunella and 2n=19-20 in yuzu were major in this investigation. The karyotyape analysis of Poncirus and orange indicated that both had 2 pairs of metacentric and 7 pairs of submetacentric chromosome, and 6 paires of Poncirus chromosome and 5 pairs of orange chromosome were possible to be identified.3.G banding and N banding method was not available for citrus chromosome. C banding method showed unique banding pattern but repeatability was very low in the present study. CMA/DAPI stain showed stable unique band in 'Tosa-Buntan' chromosome. There were 3 types of chromosome that having 2 bands, 1band and no band.

这项研究的主要目的是开发新的染色体制备方法，用于柑橘核型分析，这对于基因染色体映射很重要，第二个是使用这种新方法尝试柑橘核型分析，并开发有用的染色体带方法来鉴定柑橘染色体。 1.杰作酶促处理为120分钟。孵育2％纤维素酶 + 1％果肉酶或90分钟。育3％的胞液酶 + 2％果胶用于体外培养的植物的根尖，并进行90min。果园的年轻叶尖的果糖酶％。每个染色体中最长的染色体长度固定的4-10MUM超过2-5倍，是最长的染色体长度的2-5倍。2。poncirus和Orange中的2n = 18的染色体数量2n = 18，2n = 20-21在这项调查中，在“ Tosa-Buntan”和“ Suisho-Buntan”中，在Satsuma普通话中2n = 19，fortunella的2n = 20，在Yuzu的2n = 19-20在这项调查中是主要的。肺炎和橙色的核叶酸酯分析表明，两对元中心和7对中半脱染色体以及6副蓬孔染色体染色体和5对橙色染色体都可以识别。3.gbranding和N Banding方法不是可用于柑橘染色体。 C带方法显示出独特的带模式，但在本研究中可重复性非常低。 CMA/DAPI染色在“ Tosa-Buntan”染色体中显示出稳定的独特带。有3种具有2个带，1条带和没有频带的染色体类型。