Morphological study on the function of antigen transporting cell (ATC) and follicular dendritic cell (FDC) in the lymph node

淋巴结抗原转运细胞（ATC）和滤泡树突状细胞（FDC）功能的形态学研究

基本信息

批准号：
11670005
负责人：
YOSHIDA Toshiko
金额：
$ 2.3万
依托单位：
Toyama Medical and Pharmaceutical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

Antigen transporting cell (ATC) and follicular dendrtic cell (FDC) in the murine lymph node were investigated by electron microscopy and immunohistochemistry.1. After the immunization with two different antigens, horseradish peroxidase (HRP) and keyhole limpet hemocyanin (KLH), only HRP repeated injection induced the accumulation of large number of KLH, but no HRP on the processes of FDC in the lymph nodes. Antigen transporting cells (ATCs) located beneath the endothelial cells in the subcapsular sinus possessed both KLH and HRP.2. After elimination of the macrophages by MDPCl2 injections, MOMA- 1 positive cells disappeared in the subcapsular sinus in the lymph node. FDC-M1 positive cells remained near the subcapsular sinus and in the follicular area. Five days after the antigen injection, ATCs were presented in the MDPCl2 injected mice without MOMA-1 positive cells. ATC is different from the MOMA-1 positive cells.3. We investigated the expression of marker proteins on FDCs in popliteal lymph nodes after repeated injections of HRP into the rat footpads. The decreased accumulation of antigens in the germinal center (GMC) of lymph nodes indicated suppressed antigen exchange on FDCs after 40 antigen stimulations. S-100 protein reactions remained unchanged throughout the study, suggesting that the FDC population was preserved in GMC. On the other hand, EDS and MRC OX-2 reactions in GMC were decreased in animals given 40 injections. Decreased antigen exchange on FDC and its altered phenotype may play a role in the regulation of FDC function after repeated antigen stimuli.These experiments suggested that after antigen repeated injection, FDC was not destroyed, but may down regulate in the function. The different reaction of ATC and FDC for the antigens suggests these cells may be different type of cells.

通过电子显微镜和免疫组织化学研究了鼠淋巴结中的抗原转运细胞（ATC）和卵泡树突细胞（FDC）。1。用两种不同的抗原，辣根过氧化物酶（HRP）和钥匙孔Limpet Hemocyanin（KLH）进行免疫后，只有HRP重复注射会诱导大量KLH的积累，但在淋巴结中FDC过程上没有HRP。位于囊下窦内皮细胞下方的抗原转运细胞（ATC）具有KLH和HRP.2。通过注射MDPCL2消除巨噬细胞后，MOMA-1阳性细胞消失在淋巴结中的囊窦中。 FDC-M1阳性细胞保留在亚囊窦和卵泡区域附近。注射抗原后五天，在没有MOMA-1阳性细胞的MDPCL2注射小鼠中呈现ATC。 ATC与MOMA-1阳性细胞不同。3。在重复将HRP注射到大鼠脚垫中后，我们研究了Popliteal淋巴结中FDC上标记蛋白的表达。淋巴结生发中心（GMC）中抗原积累的减少表明在40种抗原刺激后抑制了FDC上的抗原交换。在整个研究过程中，S-100蛋白反应保持不变，这表明FDC种群保留在GMC中。另一方面，在40次注射的动物中，GMC中的ED和MRC OX-2反应减少了。反复反复的抗原刺激后，FDC上的抗原交换减少在FDC上的改变可能在FDC功能的调节中起作用。这些实验表明，在抗原重复注射后，FDC不会被破坏，但可能会在功能中调节。 ATC和FDC对抗原的不同反应表明这些细胞可能是不同类型的细胞。