Role of oxidative stress and prevention by food factors in diabetes complications

氧化应激和食物因素在糖尿病并发症中的预防作用

• 批准号: 11306009
• 负责人: OSAWA Toshihiko
• 金额: $ 24.06万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A).
• 财政年份: 1999
• 资助国家: 日本
• 起止时间: 1999 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-11306009/
• 关键词: Diabetes complication; Oxidative stress; Monoclonal antibody; Lipid hydroperoxide; Neutrophil; Cur cumin; Tetrahydrocurcumin; Diabetes cataract; ジチロシン; ヘキサノイルリジン; アキタマウス; 2型糖尿病; 免疫染色

Oxidative stress may cause free radical reactions to produce deleterious modifications in membranes, proteins, enzymes and DNA.We have developed many types of monoclonal antibodies, which are specific to oxidatively damaged lipids and proteins, especially, monoclonal antibodies specific to. hexanonyl lysine structure which is caused by the reaction linoleic acid hydroperoxide with lysine and also specific to dityrosine caused from oxidatively damaged protein by neutraphil. Using these monoclonal antibodies, we detected oxidative stress at the early stage in Akita mouse, type 2 diabetes model mouse. We also succeeded in protective role of curcumins, main yellow pigments of Curcuma longa (turmeric) in oxidative stress and diabetes catract in high-suger fed rats. Curcumin has been used widely and for a long time in the treatment of sprain and inflammation in indigenous medicine and tetrahydrocurcumin (THC) and THC was found to be produced from curcumin during absorption from the intestines, and more potent antioxidant than curcumin. By the detailed experiments, we found that THC prevents the diabetes complications much stronger than curcumin, and detailed reaction mechanisms are discussed.

氧化应激可能引起自由基反应，对膜、蛋白质、酶和DNA产生有害修饰。我们开发了多种类型的单克隆抗体，它们针对氧化损伤的脂质和蛋白质，特别是针对氧化损伤的脂质和蛋白质的特异性单克隆抗体。己壬基赖氨酸结构，是由亚油酸过氧化氢与赖氨酸反应产生的，也是中性粒细胞氧化损伤蛋白质引起的二酪氨酸特有的。使用这些单克隆抗体，我们在秋田小鼠、2 型糖尿病模型小鼠的早期阶段检测到氧化应激。我们还成功地证明了姜黄素（姜黄的主要黄色素）对高糖喂养大鼠氧化应激和糖尿病白癜风的保护作用。姜黄素在本土医学中被广泛且长期地用于治疗扭伤和炎症，四氢姜黄素（THC）和四氢姜黄素（THC）被发现是姜黄素在肠道吸收过程中产生的，是比姜黄素更有效的抗氧化剂。通过详细的实验，我们发现THC对糖尿病并发症的预防作用比姜黄素强得多，并讨论了详细的反应机制。

项目成果

Inano,H 他: "Potent preventive Action of curcumin on Radiation-induced Initiation of Mammary Tumorigenesis in Rats"Carcinogenesis. 21(10). 1835-1841 (2000)

Inano, H 等人：“姜黄素对大鼠乳腺肿瘤发生的有效预防作用”21(10) (2000)。

Nakamura, Y.etc.: "A Gutathione S-transferase Inducer from Papaya : Rapid Screening, Identification and Structure-activity Relationship of Isothiocyanates"Cancer Lett.. 157. 193-200 (2000)

Nakamura, Y.等：“来自木瓜的谷胱甘肽S-转移酶诱导剂：异硫氰酸盐的快速筛选、鉴定和结构-活性关系”Cancer Lett.. 157. 193-200 (2000)

Kato, Y.etc.: "Immunochemical Detection of Protein Dityrosine in Atherosclerotic Lesion of Apo-E-Deficient Mice Using a Novel Monoclonal Antibody"Biochem.Biophys.Res.Commun.. 275(1). 5-11 (2000)

Kato, Y.等：“使用新型单克隆抗体对 Apo-E 缺陷小鼠的动脉粥样硬化病变中的蛋白质二酪氨酸进行免疫化学检测”Biochem.Biophys.Res.Commun. 275(1)。

Kato, Y.etc.: "Preparation of Monoclonal Antibody to Ne-(Hexanonyl) lysine : Application to the Evaluation of Oxidative Modification of Rat Skeletal Muscle by Exercise with Flavonoid Supplimentation"Biochem.Biophys.Res.Commun.. 274. 389-393 (2000)

Kato, Y.等：“Ne-(己壬基)赖氨酸单克隆抗体的制备：在补充类黄酮运动对大鼠骨骼肌氧化修饰的评价中的应用”Biochem.Biophys.Res.Commun. 274. 389-

Nakamura,Y 他: "Redox Regulation of Glutathione S-transferase P l Induction by Benzyl Isothyocyanate : Possible Correlation of Enzyme Induction with the Formation of Reactive Oxygen Intermediates"Cancer Res.. 60. 219-225 (2000)

Nakamura, Y 等人：“异硫氰酸苄酯诱导的谷胱甘肽 S-转移酶 P1 的氧化还原调节：酶诱导与活性氧中间体形成的可能相关性”Cancer Res.. 60. 219-225 (2000)