Analysis and Protection on Oxidative stress in Vascular Systems

血管系统氧化应激的分析与防护

• 批准号: 06453174
• 负责人: OSAWA Toshihiko
• 金额: $ 4.61万
• 依托单位: Nagoya University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (B)
• 财政年份: 1994
• 资助国家: 日本
• 起止时间: 1994 至 1995
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06453174/
• 关键词: Oxidative stress; Lipid peroxidation; Atherosclerosis; Active oxygen spesies; 4-Hydroxynonenal; Malonaldehyde; Carboxy methyl lysime; 8-Hydroxydeoxyguanosine; アロンアルデヒド; 過酸化脂質; 4-ヒドロキシ-2-ノネナ-ル(HNE); HNE修飾タンパク質; ポリクロナール抗体; 酸化LDL

In the living system, oxidative stress is supposed to be induced by active oxygens including lipid-peroxides. In perticular, oxidative stress caused in blood systems is one of the major reason for atherosclerosis and thrombosis. Glutathione-peroxidase, catalase and superxide-dismutase play an important role for antioxidative ptotection from oxidative stress. This year, we examined the effect of lipid peroxidation products, in particular 4-hydroxynonenal on erythrocytes. Glutathione contents in erythrocytes were decreased by the reaction with 4-hydroxynonenal, and it was found that several enzymes which have SH groups such as carbonic anhydrase were found to be damaged. We have also been involved in the involvement of free radicals in diabetes. Recently we found that several biomarkers such as carboxymethyllysine (CML) and 8-hydroxydeoxyguanosine are prexcreted into urine of diabete patients. We also found that novel type of advanced glycation end products are present in blood of diabete patients, which were named as MRX and MRY.MRY and MRY can be produced by the reaction of erythrocytes of rabbit and sheep with glucose, and it was found that arginine and cysteine play an important role as the precursor of MRX and MRY.Chemical analyzes for structure elucidation is now progress.

在生命系统中，氧化应激应该是由包括脂质过氧化物在内的活性氧引起的。特别是，血液系统引起的氧化应激是动脉粥样硬化和血栓形成的主要原因之一。谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶在氧化应激的抗氧化保护中发挥着重要作用。今年，我们研究了脂质过氧化产物，特别是 4-羟基壬烯醛对红细胞的影响。与4-羟基壬烯醛的反应使红细胞中的谷胱甘肽含量降低，并且发现碳酸酐酶等几种具有SH基团的酶被破坏。我们还研究了自由基与糖尿病的关系。最近我们发现一些生物标志物如羧甲基赖氨酸（CML）和8-羟基脱氧鸟苷预先排泄到糖尿病患者的尿液中。我们还发现糖尿病患者的血液中存在新型晚期糖基化终产物，分别命名为MRX和MRY。MRY和MRY可由兔和羊的红细胞与葡萄糖反应产生，并发现：精氨酸和半胱氨酸作为MRX和MRY的前体发挥着重要作用。用于结构阐明的化学分析现已取得进展。

项目成果

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Tsuda,T.: "Antioxidative Pigments Isolated from the Seeds of Phaseolus vulgaris L." J.Agric.Food Chem.42. 248-251 (1994)

Tsuda,T.：“从菜豆种子中分离出的抗氧化色素”。