Generation of Chlamydia-induced atherosclerosis model mice

衣原体诱导的动脉粥样硬化模型小鼠的产生

• 批准号: 10680774
• 负责人: YAGAMI Kenichi
• 金额: $ 1.73万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10680774/
• 关键词: hypertensive mice; chlamydia pneumoniae; atherosclerosis; Chlamydia pneumoniae; クラミジア; マウス

Pathgenesis of vascular remodeling in hypertensive transgenic mice and infectivity of Chlamydia pneumoniae to mice were examined to generate atherosclerotic disease model mice.1) The hypertensive transgenic mice carrying the human renin and human angiotensinogen gene progressively developed remarkable vascular hypertrophy composed of dedifferentiation of vascular smooth muscule cells and extracellular matrix accumulation in the thoracic aorta. The hyperplasia was predominant in the abdominal aorta. The hypertensive transgenic mice and C57BL/6 control mice fed with atherogenic diet for 3 months were compared in atherosclerotic lesion in aorta. Accelerated atherosclerotic lesion was demonstrated in the hypertensive transgenic mice.2) Infectivity of C. pneumoniae to mice from intranasal route was demonstrated in C57BL/6 mice by detection of antibody and PCR. Infected mice demonstrated no symptom during 3 months after infection. Atherosclerotic lesion was demonstrated in the hypertensive transgenic mice infected with C. pneumoniae.

检查了血管重塑在高血压转基因小鼠中的途径以及肺炎氯化肺炎对小鼠的感染性，以产生动脉粥样硬化疾病模型的小鼠。1）携带人类肾素和人类血管紧张基因基因的高血压转基因小鼠，逐渐发育出了良好的血管素基因群，由血管紧张的平稳性繁殖群体逐渐成分，这些均具有良好的血管性繁殖，这些平稳性繁殖群是血管的平稳性，这些平稳性无效。在胸主动脉中积累。腹主动脉中的增生占主导地位。在主动脉的动脉粥样硬化病变中比较了三个月的高血压转基因小鼠和C57BL/6对照小鼠3个月。在高血压转基因小鼠中证明了加速的动脉粥样硬化病变。2）通过检测抗体和PCR，在C57BL/6小鼠中证明了肺炎梭状芽胞杆菌向小鼠的感染性。在感染后3个月内，感染的小鼠没有症状。在感染肺炎梭菌的高血压转基因小鼠中证明了动脉粥样硬化病变。

项目成果

Nishijo N.et al.: "Vascular remodeling in hypertensive transgenic mice."Exp Anim. 48(3). 203-208 (1999)

Nishijo N.等人：“高血压转基因小鼠的血管重塑。”Exp Anim。

Nishijo N.,Yagami K.et al.: "Vascular remodeling in hypertensive transgenic mice"Exp. Anim.. 48. 203-208 (1999)

Nishijo N.、Yagami K.等人：“高血压转基因小鼠的血管重塑”实验。

Nishijo N.,Yagami K.et al.: "Salt-sensitive aortic aneurysm and rupture in hypertensive transgenic mice that overproduce angiotensin II"Lab. Invest.. 78. 1059-1066 (1998)

Nishijo N.、Yagami K.等人：“过量产生血管紧张素 II 的高血压转基因小鼠中的盐敏感性主动脉瘤和破裂”实验室。

Nishijo N., Yagami K et al.: "Vascular remodeling in hypertensive transgenic mice"Exp. Anim.. 48. 203-208 (1999)

Nishijo N.、Yagami K 等人：“高血压转基因小鼠的血管重塑”实验。

Nishijo N., Yagami K et al.: "Salt-sensitive aortic aneurysm and rupture in hypertensive transgenic mice that overproduce angiotensin II"Lab. Invest.. 78. 1059-1066 (1998)

Nishijo N.、Yagami K 等人：“过量产生血管紧张素 II 的高血压转基因小鼠中的盐敏感性主动脉瘤和破裂”实验室。