Development of Transgenic Mice to Visualize Tumor Tissues

培育可视化肿瘤组织的转基因小鼠

• 批准号: 23650228
• 负责人: YAGAMI Kenichi
• 金额: $ 2.41万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-23650228/
• 关键词: E G FP; パルボウイルス; 発癌; ＥＧＦＰ; 発がん; EGFP; E G FP; パルボウイルス; 発癌; ＥＧＦＰ; 発がん; EGFP

The present study is aimed to develop the transgenic mouse that express reporter gene, EGFP, under the parvovirus P4 promoter, and to visualize tumor by in vivo imaging. The P4-EGFP Tg mice older than 20-days old expressed EGFP in spermatocytes of testis, but not in other tissues. Some of tumor tissues in P4-EGFP mice induced with carcinogen or mating to oncogenic ras H2 Tg mice, were detectable by EGFP expression.

本研究的目的是开发在细小病毒P4启动子下表达记者基因EGFP的转基因小鼠，并通过体内成像可视化肿瘤。超过20天的P4-EGFP TG小鼠在睾丸的精子细胞中表达了EGFP，但在其他组织中却没有。可以通过EGFP表达检测到用致癌物或致癌Ras H2 TG小鼠交配的P4-EGFP小鼠中的一些肿瘤组织。