Effect of endurance training on accumulation of age-associated mitochondrial DNA deletions in old rats.

耐力训练对老年大鼠年龄相关线粒体 DNA 缺失积累的影响。

基本信息

批准号：
10680015
负责人：
KUNO Shinya
金额：
$ 2.11万
依托单位：
University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

It has been proposed that age-associate decline of muscle metabolism of energy product are resulted from the accumulation of mitochondrial DNA (mtDNA) deletions in several species (Linnane et al., 1989). However, age-associated mt DNA deletions have not been successfully detected in the rat skeletal muscles (Filser et al., 1997). Exercise has been known to improve the muscle metabolism and then may have a benefit to prevent the accumulation of mtDNA deletions with age. As long as we know, little has been known about the effects of exercise on the accumulation of mtDNA deletions so far. The purpose of this study was to detect the age-associated mtDNA deletions in the rat skeletal muscles, and to examine whether endurance training effects on the accumulation of mtDNA deletions with age in different type of skeletal muscle. We used forty five Wistar male rats (10 wks : n=29, 90 wks : n=16) in this study. Young rats were divided into three groups (20 m/min training group, 30 m/min training … More group and control group) and old rats were divided into two groups (20 m/min training group and control group). The training protocol consisted of 60 min, 5 days/wk for 10 wks. MtDNA of m. soleus (SOL) and m.plantaris (PLA) were analyzed by polymerase chain reaction (PCR). The presence of age-associated 4.8-kb deletion (common deletion) and the number of deletion products detected per rat were examined. The presence of common deletion was evaluated by the number of rats in each group and the number of deletion was estimated by counting the deletion products visualized per rat. Two of the young rats (6.9%) and nine of the old rats (56.3%) had common deletion in SOL, while two of the young rats (6.9%) and fifteen of the old rats (93.8%) had in PLA. No effect of the endurance training on the presence of common deletion was seen in either the young group or the old group. In the old group, the presence of common deletion was higher in PLA than that in SOL. The numbers of deletion in PLA increased with age significantly (p<0.01). However the numbers of deletion in SOL showed no significant difference between the young and the old groups. The training effect on the numbers of deletion was not observed in any groups. In the young group, the number of mtDNA deletion products in SOL was higher than that in PLA. In the old group, however, the value was lower in SOL than in PLA.In conclusion, we could detect the accumulation of age-associated mtDNA deletions of the rat skeletal muscles, and observed no endurance training effect on that in this study. Moreover, the accumulation of mtDNA deletions can be specific according to fiber types. Less

能量产物的肌肉代谢是由几个物种的肌肉代谢的肌肉代谢。骨骼肌肉（Filser等人，1997年）。在大鼠骨骼肌肉中，在这项研究中，在不同类型的骨骼雄性大鼠中，终端不足训练对MTDNA缺失的积累是否影响（20 m/min训练组，30m/min训练……更多的组和对照组。通过聚合酶链雷（PCR）分析），研究了每只大鼠检测到的脱落产物的数量。通过计算每个r可视化的删除产物（6.9％）和九个老鼠（56.3％）的删除产物（56.3％）在SOL中删除，而两只年轻大鼠（6.9％）大鼠（93.8％）在旧组中看到了PLA，在旧组中，在sol中存在常见的脱落。在旧组中，在旧组中观察到的缺失数量不高。 - 在这项研究中，大鼠骨骼肌肉训练的效果相关的mtDNA缺失