Measurement of cerebral inhibitory neuronal receptors with positron emission tomography in relation to neuronal injury.

用正电子发射断层扫描测量脑抑制性神经元受体与神经元损伤的关系。

基本信息

批准号：
10671287
负责人：
NARIAI Tadashi
金额：
$ 2.05万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671287/
关键词：
PET Receptors adenosine benzodiazepine cerebral ischemia neurotrauma cerebral blood flow cats ネコ脳血流

项目摘要

Eighteen adult cats were intubated and anesthetized with 1.5% isoflurane, and were held by plastic ear bars to obtain a reproducible head position. In thirteen cats, right MCA was exposed with transorbital approach and was ready to be occluded with a microvascular clip. CBF before and during occlusion of MCA, and that immediately after reperfusion was quantified with i.v. bolus injection of ィイD115ィエD1O labeled water (200MBq per cat) and a PET instrument for animal scan, SHR2000 (Hamamatsu photonics, FWHM=4mm). Adenosine A1 receptor binding potential, central benzodiazepine receptor binding potential and CMRglc was serially quantified with 11C-MPDX (200MBq), 11C-flumazenil (200MBq) 18F-FDG (100 MBq) respectively. All PET parameters were presented as the right (ischemic)/left (control) ratio. The data of ischemic cat was compared with that of normal cats (N=5). Degree of ischemic damage was evaluated by histological evaluation and/or by T2 weighted MR image with animal MRI system (Unity … More plus, Varian). Four cats did not survive the 1st day because of brain edema and were classified as most severe ischemic insult. Three cats survived and had the infarction both in cortex and striatum. The other six had infarcted lesion only in striatum. CBF during occlusion of MCA was significantly reduced in all 3 ischemic group than in normal group. However, no statistically significant difference was found among the ischemic groups. Right/Left ratio of CBF after reperfusion and that of FDG uptake did not show any statistically significant difference among all groups. Adenosine A1 receptor binding potential and benzodiazepine receptor binding potential were significantly reduced in group with severe ischemic insult than that with normal or milder insult. Of two receptors examined, reduced binding potential of adenosine A1 receptor was more sensitive to the degree of ischemic insult. In this study, the degree of decreased binding of adenosine A1 receptor after reperfusion and the degree of ischemic insult correlated well. PET imaging of cerebral adenosine receptor with C-11 MPDX can be a suitable in vivo imaging technique to evaluate the function of adenosine and adenosine receptor in relation to cerebral ischemia. Less

十八只成年猫被插管并用1.5％的异氟烷进行麻醉，并由塑料耳杆固定，以获得可重现的头部位置，右MCA持续了透射术方法，并准备用微血管夹遮住。在闭塞过程中，MCA和在动物扫描的宠物仪器中进行了定量，SHR2000（Hamamatsu Photonics，FWHM = 4mm） 11C-MPDX（200MBQ），11c-flumazenil（200MBQ）18F-FDG（100 MBQ）分别以右（缺血）/左（对照）的比率显示了所有PET参数。正常的猫（n = 5）。在纹状体中，MCA的梗塞仅在CBF期间显着降低了缺血组。 A1受体结合势和苯二氮卓的结合电位显着降低了SAISE IC的侮辱，即在检查正常或降低的症状，腺苷A1 Egree的结合减少了腺苷A1受体的脱脂症。侮辱性的。与C-11 MPDX的大脑腺苷受体的老化是一种适合评估腺苷和腺苷受体相对于脑缺血的功能的合适的体内成像技术