Experimental use of positron emission tomography to analyze the damage of neuronal circuit and its restration with cell transplantation therapy.

实验利用正电子发射断层扫描分析神经元回路的损伤及其细胞移植治疗的恢复。

基本信息

批准号：
17591504
负责人：
NARIAI Tadashi
金额：
$ 2.11万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We have established an experimental system with combined use of, 1) in vivo micro-PET study, 2) in vitro brain slice PET study, and 3) clinical human PET study to investigate the mechanism of neuronal damage and the process of functional regeneration by therapeutic maneuver such as neuronal transplantation. Results of several research topics has been summarized as below.1) We have investigated the dopaminergic function of animal model of Parkinson's disease using animal PET camera. We also treated these animals with the implantation of fatal nigral dopaminergic cells and followed the time course of functional recovery of each animals using PET. Such experimental system is highly efficient as we can eliminate the inter-individual variance of experimental animals. We could also establish a practical system of using micro-PET camera, that was introduced for the first time in our country, for general experimental use.2) Using the same experimental protocol as above, we published a paper to … More compare the degree of functional recovery of experimental animals with the function of dopaminergic neuronal transduction evaluated with animal PET. This is the first to report of quantitative comparison between behavior and receptor function in animal study.3) We have started an PET experiment to evaluate the function of microglia in relation to neuronal damage by ischemia and trauma. A suitable positron labeled radioligand to map the peripheral type benzodiazepine receptor was introduced for this experiment. Quantitative measurement of animal behavior after ischemia or trauma was performed to compare them with the receptor binding measured with animal PET camera.4) We have developed a novel research technique to visualize the neuronal conduction in incubated brain slices of experimental animal. Optical imaging with fluorescent dye to detect neuronal activity and the in-vitro PET technique to measure glucose metabolism was applied for this system. This system was also used for the analysis of pathology of human brain that was resected from patients with intractable epilepsy.5) We also applied various PET technique for the analysis of human brain damages elicited by ischemia, tumor, epilepsy or trauma etc. These data was pooled for statistical analysis to investigate the functional damage and the process of recovery by therapeutic procedures. Such database can be used for future study to evaluate the recovery of human brain function by newly developed regeneration therapy. Less

我们已经建立了一个实验系统，共同使用了1）体内微PET研究，2）体外脑切片PET研究，以及3）临床人类PET研究，以研究神经元损伤的机制和通过治疗性手术的功能再生过程，例如神经元移植。几个研究主题的结果总结如下1）我们已经使用动物宠物相机研究了帕金森氏病动物模型的多巴胺能功能。我们还用致命的ni虫多巴胺能细胞植入了这些动物，并遵循了使用PET对每种动物进行功能恢复的时间过程。这种实验系统非常有效，因为我们可以消除实验动物的个体间差异。我们还可以建立一个使用Micro-PET摄像机的实用系统，该系统是在我国首次引入的，用于一般实验用途。2）使用与上述相同的实验协议，我们发表了一篇论文，以相比……更多地比较了实验动物的功能恢复程度与多巴胺能神经元翻译的功能。这是动物研究中第一个报告行为和受体功能之间定量比较的报道。3）我们已经开始了一个PET实验，以评估小胶质细胞与缺血和创伤相对于神经元损害的功能。为此实验引入了一个合适的标有放射线的标记辐射体，用于绘制外周型苯二氮卓受体。进行缺血或创伤后动物行为的定量测量与动物宠物摄像机测量的受体结合进行了比较。4）我们开发了一种新型的研究技术，以可视化实验动物孵育的脑切片中的神经元传导。使用荧光染料进行光学成像，用于检测神经元活性和测量葡萄糖代谢的体外PET技术。该系统还用于分析从患有顽固性癫痫的患者中切除的人类大脑病理学。5）我们还应用了各种PET技术来分析缺血，肿瘤，癫痫或创伤等人的人脑损害。这些数据汇集了这些数据以进行统计分析，以研究通过治疗程序的功能损害和恢复过程。这种数据库可用于未来的研究，以评估新开发的再生疗法的人脑功能的恢复。较少的