Experimental use of positron emission tomography to analyze the damage of neuronal circuit and its restration with cell transplantation therapy.

实验利用正电子发射断层扫描分析神经元回路的损伤及其细胞移植治疗的恢复。

基本信息

批准号：
17591504
负责人：
NARIAI Tadashi
金额：
$ 2.11万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

项目摘要

We have established an experimental system with combined use of, 1) in vivo micro-PET study, 2) in vitro brain slice PET study, and 3) clinical human PET study to investigate the mechanism of neuronal damage and the process of functional regeneration by therapeutic maneuver such as neuronal transplantation. Results of several research topics has been summarized as below.1) We have investigated the dopaminergic function of animal model of Parkinson's disease using animal PET camera. We also treated these animals with the implantation of fatal nigral dopaminergic cells and followed the time course of functional recovery of each animals using PET. Such experimental system is highly efficient as we can eliminate the inter-individual variance of experimental animals. We could also establish a practical system of using micro-PET camera, that was introduced for the first time in our country, for general experimental use.2) Using the same experimental protocol as above, we published a paper to … More compare the degree of functional recovery of experimental animals with the function of dopaminergic neuronal transduction evaluated with animal PET. This is the first to report of quantitative comparison between behavior and receptor function in animal study.3) We have started an PET experiment to evaluate the function of microglia in relation to neuronal damage by ischemia and trauma. A suitable positron labeled radioligand to map the peripheral type benzodiazepine receptor was introduced for this experiment. Quantitative measurement of animal behavior after ischemia or trauma was performed to compare them with the receptor binding measured with animal PET camera.4) We have developed a novel research technique to visualize the neuronal conduction in incubated brain slices of experimental animal. Optical imaging with fluorescent dye to detect neuronal activity and the in-vitro PET technique to measure glucose metabolism was applied for this system. This system was also used for the analysis of pathology of human brain that was resected from patients with intractable epilepsy.5) We also applied various PET technique for the analysis of human brain damages elicited by ischemia, tumor, epilepsy or trauma etc. These data was pooled for statistical analysis to investigate the functional damage and the process of recovery by therapeutic procedures. Such database can be used for future study to evaluate the recovery of human brain function by newly developed regeneration therapy. Less

我们建立了一个综合使用1）体内显微PET研究、2）体外脑切片PET研究和3）临床人体PET研究的实验系统，以研究神经元损伤的机制和功能再生的过程。几个研究课题的结果总结如下：1）我们使用动物PET相机研究了帕金森病动物模型的多巴胺能功能。黑质多巴胺能细胞，并使用 PET 跟踪每只动物的功能恢复时间过程，这样的实验系统是高效的方差，因为我们可以消除实验动物的个体间差异，我们还可以建立一个使用微型 PET 相机的实用系统。国内首次引进，用于一般实验用途。2）使用与上述相同的实验方案，我们发表了一篇论文，以比较实验动物的功能恢复程度和评估的多巴胺能神经元转导功能与动物PET。这是第一个在动物研究中定量比较行为和受体功能的报告。3）我们已经开始了一项PET实验，以评估小胶质细胞与缺血和创伤引起的神经元损伤的功能。该实验引入了外周型苯二氮卓受体图，对缺血或创伤后的动物行为进行了定量测量，以将其与动物 PET 相机测量的受体结合进行比较。4) 我们开发了一种新颖的研究技术来可视化该受体。该系统还使用荧光染料光学成像来检测神经元活动，并使用体外 PET 技术来测量葡萄糖代谢。 5）我们还应用各种PET技术来分析缺血、肿瘤、癫痫或外伤等引起的人脑损伤。将这些数据汇总进行统计分析，以研究功能损伤以及通过治疗程序恢复的过程，该数据库可用于未来的研究，以评估新开发的再生疗法对人类大脑功能的恢复情况。