Investigation for the machamism of hipocampal damage and itsu protection using in vivo and in vitro positron emission tomography study of human brain.

利用人脑体内外正电子发射断层扫描研究研究海马损伤的机制及其保护。

基本信息

批准号：
14571301
负责人：
NARIAI Tadashi
金额：
$ 2.24万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

1) All the patients with intractable temporal lobe epilepsy (TLE) who were operated on in Department of Neurosurgery, Tokyo Medical and Dental University underxvent measurement of cerebral metabolic rate of glucose and the binding potential of central benzodiazepine receptors with positron emission tomography (PET). A new project to image the cerebral adenosine Al receptor using newly developed tracer C-il MPDX and PET in patients with TLE.2) In this new project, we revealed that the adenosine receptor decreased in the fugiform gyrus of focus side and increased in the frontal cortex of focus side. This new finding is now prepared for a paper and a presentation in the international meeting.3) Investitaion to measure the cerebral metabolism of human cortical slice which were obtained in 10 patients with TLE was completed. In this analysis, ~ve revealed that the metabolism in the kalium obtained media corresponded with the metabolism in the preoperative condition measured with PET but that in the control media did not. It suggested that the cerebral metaboliosm in so to speak resting condition is the state that neuron, is working with full neuronal input. This finding is now repared for a paper and a presentation in the international meeting.4) We performed the comparative experiment between the metabolism and neuronal electrical activity in human slice. The latter was performed using optical imaging technique. This experiment revealed that the metabolism is induce by potassium stimulation even in the condition where neuronal conduction is not established. This finding suggest the different behavior of neuron in the slice condition.

1）所有在神经外科，东京医学和牙科大学在葡萄糖脑外代谢率和苯并二氮卓受体中部与正面发射术（PET）的结合潜力的测量的患者都在神经外科，东京医学和牙齿大学中进行操作的所有患者。一个新的项目，使用新开发的示踪剂C-IL MPDX和PET对大脑腺苷AL受体进行成像。现在，这一新发现已准备好在国际会议上进行纸张和演讲。3）投资衡量人类皮质切片的脑代谢，这是10名TLE患者获得的。在此分析中，〜ve揭示了Kalium获得的培养基中的代谢与用PET测量的术前疾病中的代谢相对应，但在对照培养基中却没有。它表明，在说静止状态的大脑代谢是神经元正在使用完整的神经元输入的状态。现在，这一发现是据论文和国际会议上的演讲予以予以的。4）我们在人类切片中的新陈代谢和神经元电活动之间进行了比较实验。使用光学成像技术进行后者。该实验表明，即使在没有建立神经元传导的情况下，钾刺激也会诱导代谢。这一发现表明在切片条件下神经元的行为不同。