Investigation for the machamism of hipocampal damage and itsu protection using in vivo and in vitro positron emission tomography study of human brain.

利用人脑体内外正电子发射断层扫描研究研究海马损伤的机制及其保护。

基本信息

批准号：
14571301
负责人：
NARIAI Tadashi
金额：
$ 2.24万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

项目摘要

1) All the patients with intractable temporal lobe epilepsy (TLE) who were operated on in Department of Neurosurgery, Tokyo Medical and Dental University underxvent measurement of cerebral metabolic rate of glucose and the binding potential of central benzodiazepine receptors with positron emission tomography (PET). A new project to image the cerebral adenosine Al receptor using newly developed tracer C-il MPDX and PET in patients with TLE.2) In this new project, we revealed that the adenosine receptor decreased in the fugiform gyrus of focus side and increased in the frontal cortex of focus side. This new finding is now prepared for a paper and a presentation in the international meeting.3) Investitaion to measure the cerebral metabolism of human cortical slice which were obtained in 10 patients with TLE was completed. In this analysis, ~ve revealed that the metabolism in the kalium obtained media corresponded with the metabolism in the preoperative condition measured with PET but that in the control media did not. It suggested that the cerebral metaboliosm in so to speak resting condition is the state that neuron, is working with full neuronal input. This finding is now repared for a paper and a presentation in the international meeting.4) We performed the comparative experiment between the metabolism and neuronal electrical activity in human slice. The latter was performed using optical imaging technique. This experiment revealed that the metabolism is induce by potassium stimulation even in the condition where neuronal conduction is not established. This finding suggest the different behavior of neuron in the slice condition.

1）东京医科齿科大学神经外科手术的所有难治性颞叶癫痫（TLE）患者均未采用正电子发射断层扫描（PET）测量脑葡萄糖代谢率和中枢苯二氮卓受体结合电位。使用新开发的示踪剂 C-il MPDX 和 PET 对 TLE 患者的脑腺苷 A1 受体进行成像的新项目。2) 在这个新项目中，我们发现腺苷受体在焦点侧的毛状回中减少，在焦点侧的毛状回中增加。焦点侧的额叶皮层。这一新发现现已准备好在国际会议上发表论文和报告。3) 对从 10 名 TLE 患者获得的人体皮质切片进行脑代谢测量的研究已经完成。在该分析中，我们发现获得钾的培养基中的代谢与 PET 测量的术前条件下的代谢相符，但对照培养基中的代谢却不然。它表明，可以说静息状态下的大脑代谢是神经元在完全神经元输入的情况下工作的状态。这一发现现已在国际会议上发表论文和演讲。4）我们在人体切片中进行了新陈代谢和神经元电活动的比较实验。后者是使用光学成像技术进行的。该实验表明，即使在神经元传导未建立的情况下，钾刺激也会诱导新陈代谢。这一发现表明神经元在切片条件下的不同行为。