Effects of stretch on cell proliferattion of fibroblast and collagen metabolism

拉伸对成纤维细胞增殖和胶原代谢的影响

基本信息

批准号：
10670782
负责人：
ITO Masaaki
金额：
$ 2.3万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

Although dermal fibroblasts receive stretch and relaxation exerted by muscle movement, biological effects of the mechanical force on the cells remains unclear. In the present study to assess effects of stretch and relaxation on cell proliferation and myofibroblast differentiation of dermal fibroblasts, cells were loaded in a biaxial strain device, which was controlled with computer. Cells were subjected to cyclic sigmoidal stretch and relaxation six cycles/min with 10% maximum change. Cell number was increased in strained cells compared with non-strained ones, and transforming growth factor-β1(TGF-β1)showed an additional effect to the strain-induced stimulation of cell proliferation. Immunoblot assay showed that level of α-smooth muscle actin(SMA)expression of cells , either enhanced by TGF-β1 or not, was decreased by cyclic strain. Flow cytometry suggested that all cells, either stimulated with TGF-β1 or not, decrease the SMA expression in response to strain. Immunofluorescence microscopy showed that there were star-like, spreaded cells that were highly positive for SMA and spindle cells that were negative or slightly positive for SMA, and that a ratio of the former cells to the latter ones was decreased in response to the cyclic strain. Conditioned medium form cells subjected to the cyclic strain had no effects on both cell proliferation and SMA expression of non-strained cells, indicating that the strain-induced effects are not medicated through autocrine mechanism. Genistein abolished both the stimulatory effect of strain on cell proliferation and its suppressive effect on SMA expression, whereas staurosporine or pertussistoxin did not. These data indicatethat the strain-induced effects on both cell proliferation and SMA expression are mediated by tyrosine kinase-dependent mechanism.

尽管肌肉运动在礼物中不清楚的肌肉运动不清楚皮肤成纤维细胞和松弛，以评估细胞刺激性和肌纤维细胞衍射外成纤维细胞的放松，但将CE lls加载到双轴菌株中，但会使细胞延伸。六个循环/分钟有10％m的变化。细胞的SMA表达，是否通过TGF-β1增强，通过环状应变流式细胞仪降低，表明细胞表明细胞是恒星状的，散布的细胞，对SMA和螺旋细胞高阳性和螺旋细胞为阴性或负阴性细胞。 SMA略有阳性，而对循环菌株的EL响应于循环菌株的EL降低了后者的前一个细胞的阳性。 - 诱导的，不是细胞泛滥的the theT分泌机制，其抑制性表达对细胞泛滥和表达的影响均由酪氨酸激酶依赖性机制介导。