Investigation of the Relationship between Invasion of Fungi and Alteration of Cell Kinetics in Hair Apparatus in Experimental Dermatophytosis, Especially in Comparison with Human Hair Fungal Infection

实验性皮肤癣菌病中真菌侵袭与毛发装置细胞动力学改变之间关系的研究，特别是与人类毛发真菌感染的比较

基本信息

批准号：
07670933
负责人：
ITO Masaaki
金额：
$ 1.28万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

It is well known that the experimental Trichophyton (T) dermatophytosis produced in hairy animals shows natural healing within a few weeks, but human dermatophytosis caused by T is usually apt to be uncurable without therapy. In the present study, in order to elucidate the process of the natural healing of the experimental dermatophytosis, a strain of T mentagrophytes was inoculated in the guinea pig back skin by a wet disc method and the skin lesions were successively biopsied and examined. Histologically, inflammatory changes in the epidermis and upper dermis reached the maximal state on day 14. Most of the hair apparatus began to retract their hair bulbs on day 16. It was shown by periodic acid-Schiff staining that the infected fungi intruded down into the keratinizing zone of the hair cortex, although few inflammatory cells infiltrated around the lower part of the hair apparatus. By BrdU staining, BrdU-labeling indices (LI) in the hair bulbs as well as in the basal layrs of the epi … More dermis and follicular epithelia significantly increased on day 14. Subsequently, the BrdU-LI became lower and lower with retraction of hair bulbs. From these observations, although hyperproliferation of the epidermis is a factor for the natural healing of the experimental dermatophytosis, it is strongly suggested that fungal infection may first evoke proliferative changes in the hair bulbs as well as in the epidermis, and, then, induce retraction of the hair bulbs and falling away of hairs, resulting in complete elimination of the infectinf fungi. The infecting fungi appear to directly affect the cell kinetics in hair bulbs. On the other hand, in human dermatophytosis caused by various kinds of T,especially when the hair apparatus is affected, the fungi invade and intrude into the keratinizing zone of the hair cortex ; however, the hair apparatus seldom shows retraction of the hair bulb, although the produced hair becomes atrophic (Arch Dermatol Res 283 : 233,1991). If it is assuming that the ability of tissue injury by T is the same both in animal and human skin tissues, the contradictious findings of animal and human hair apparatus infected by T may be due to structural differences, such as difference in size of hair bulb, differences in cell kinetics in hair bulbs, including hair cycle, and/or difference in tolerance of hair bulbar cells to the poison from the fungi, between animals and human beeings. Less

众所周知，在毛茸茸的动物中产生的实验性滴虫（T）皮肤植物学在几周内显示出自然的愈合，但是由T引起的人类皮肤植物学通常是不可育的，没有治疗。在本研究中，为了阐明实验性皮肤植物学的自然愈合过程，通过湿盘方法在豚鼠后皮肤中接种了T脑植物的菌株，并将皮肤病变连续进行活检和检查。从组织学上讲，表皮和上真皮的炎症变化在第14天达到了最大状态。大多数头发设备开始在第16天开始缩回其头发鳞茎。周期性酸 - 雪奇染色表明，感染的真菌侵入了炎症细胞的角膜细胞，但在较少的炎症细胞中侵入了较少的炎症细胞，较少的炎症细胞的一部分是较低的一部分。通过BRDU染色，毛灯泡以及EPI的基本底层中的Brdu标记指数（LI）……在第14天，更多的真皮和卵泡上皮显着增加。随后，Brdu-Li随着毛囊的缩回而变得越来越降低。从这些观察中，尽管表皮的过度增殖是实验性皮肤植物学病自然愈合的一个因素，但强烈建议，真菌感染可能首先引起毛泡中的增殖剂的变化，以及在表皮中，然后诱导毛泡和掉落的毛发恢复，从而使毛发不见了，从而恢复了不见了。感染真菌似乎直接影响毛鳞茎中的细胞动力学。另一方面，在由各种T的人类皮肤植物学作用中，尤其是当发型仪器受到影响时，真菌侵入并侵入了毛皮皮质的角化区。然而，尽管产生的头发变成萎缩，但头发设备很少显示出发灯泡的回缩（Arch Dermatol Res 283：233，1991）。如果假设在动物和人类皮肤组织中，T组织损伤的能力都是相同的，那么受T感染的动物和人毛发仪的矛盾发现可能是由于结构差异所致，例如毛状剂量的大小差异，细胞动力学的细胞动力学差异，包括毛发循环的植物和/或人类毒药的差异。较少的