Investigation of the Relationship between Invasion of Fungi and Alteration of Cell Kinetics in Hair Apparatus in Experimental Dermatophytosis, Especially in Comparison with Human Hair Fungal Infection

实验性皮肤癣菌病中真菌侵袭与毛发装置细胞动力学改变之间关系的研究，特别是与人类毛发真菌感染的比较

基本信息

批准号：
07670933
负责人：
ITO Masaaki
金额：
$ 1.28万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

项目摘要

It is well known that the experimental Trichophyton (T) dermatophytosis produced in hairy animals shows natural healing within a few weeks, but human dermatophytosis caused by T is usually apt to be uncurable without therapy. In the present study, in order to elucidate the process of the natural healing of the experimental dermatophytosis, a strain of T mentagrophytes was inoculated in the guinea pig back skin by a wet disc method and the skin lesions were successively biopsied and examined. Histologically, inflammatory changes in the epidermis and upper dermis reached the maximal state on day 14. Most of the hair apparatus began to retract their hair bulbs on day 16. It was shown by periodic acid-Schiff staining that the infected fungi intruded down into the keratinizing zone of the hair cortex, although few inflammatory cells infiltrated around the lower part of the hair apparatus. By BrdU staining, BrdU-labeling indices (LI) in the hair bulbs as well as in the basal layrs of the epi … More dermis and follicular epithelia significantly increased on day 14. Subsequently, the BrdU-LI became lower and lower with retraction of hair bulbs. From these observations, although hyperproliferation of the epidermis is a factor for the natural healing of the experimental dermatophytosis, it is strongly suggested that fungal infection may first evoke proliferative changes in the hair bulbs as well as in the epidermis, and, then, induce retraction of the hair bulbs and falling away of hairs, resulting in complete elimination of the infectinf fungi. The infecting fungi appear to directly affect the cell kinetics in hair bulbs. On the other hand, in human dermatophytosis caused by various kinds of T,especially when the hair apparatus is affected, the fungi invade and intrude into the keratinizing zone of the hair cortex ; however, the hair apparatus seldom shows retraction of the hair bulb, although the produced hair becomes atrophic (Arch Dermatol Res 283 : 233,1991). If it is assuming that the ability of tissue injury by T is the same both in animal and human skin tissues, the contradictious findings of animal and human hair apparatus infected by T may be due to structural differences, such as difference in size of hair bulb, differences in cell kinetics in hair bulbs, including hair cycle, and/or difference in tolerance of hair bulbar cells to the poison from the fungi, between animals and human beeings. Less

众所周知，在多毛动物中产生的实验性毛癣菌（T）皮肤癣菌病在几周内就会自然愈合，但由毛癣菌引起的人类皮肤癣菌病通常在不治疗的情况下往往无法治愈。本研究旨在阐明这一过程。实验性皮肤癣菌病自然愈合的研究，采用湿盘法将一株须癣菌接种于豚鼠背部皮肤，成功治愈皮损组织学检查，表皮和真皮上层的炎症变化在第14天达到最大状态。大多数毛器在第16天开始回缩毛球。过碘酸希夫染色显示感染的真菌。通过 BrdU 染色，尽管很少有炎症细胞浸润到头发皮质的角化区，第 14 天，毛球以及真皮基底层和毛囊上皮中的 BrdU 标记指数 (LI) 显着增加。随后，随着毛球的回缩，BrdU-LI 变得越来越低。这些观察结果表明，尽管表皮过度增殖是实验性皮肤癣菌病自然愈合的一个因素，但强烈表明真菌感染可能首先引起毛球的增殖性变化另一方面，感染真菌似乎直接影响毛球的细胞动力学。、各种T引起的人体皮肤癣菌病，尤其是毛器受累时，真菌侵入并侵入毛皮质角化区，但毛器很少表现出来；毛球回缩，尽管产生的毛发变得萎缩（Arch Dermatol Res 283：233，1991）如果假设 T 损伤组织的能力在动物和人类皮肤组织中是相同的，则矛盾的发现。动物和人类的毛器被 T 感染可能是由于结构差异，例如毛球大小的差异、毛球细胞动力学的差异（包括毛发周期）和/或毛球细胞对毛球耐受性的差异。动物和人类之间的真菌毒物较少。