Mechanism of toxin synthesis using cultured poisonous mushroom

人工培养毒蘑菇合成毒素的机制

• 批准号: 10670380
• 负责人: GONMORI Kunio
• 金额: $ 2.3万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10670380/
• 关键词: Mushroom poisoning; Cultivation; Magic mushroom; Growth factor; Analysis; HPLC; 毒きのこ; 幻覚きのこ; 毒成分

Several kind of mushrooms has strong toxicity to kill people. However, there have been only few reports of toxicological analysis on poisonous mushroom in forensic field. This study aimed to cultivate some poisonous mushrooms for the purpose of investigation and research in forensic toxicology. In our experiment the following mushrooms were cultivated, Pleurotus ostreatus, Lentinus edodes, Psilocybe cubensis, Amanita virosa, Lampteromyces japonicus and Gymnopilus spectabilis. Eventually the cultivation of Lyophyllum and Psilocybe cubensis resulted well-grown mushrooms. In the case of the Psilocybe cubensis, psilocybin and psilocin are the main hallucinogenic components. We observed the effect of storage on the concentration of these two hallucinogens. From harvesting the Psilocybe cubensis the freezing method provided the most valuable results to store mushrooms. A sample from a poisonous case suggested magic mushroom ingestion was analyzed. The mushroom was described as Psilocybe subcubensis. Recently the interest of the magic mushroom poisoning appears to be growing around the world. This research attempts to promote the investigation protocol for magic mushroom's cases.

有几种蘑菇具有很强的毒性，可以致人死亡。然而，法医学领域对毒蘑菇毒理学分析的报道却很少。本研究旨在培育一些有毒蘑菇，以供法医毒理学调查和研究之用。在我们的实验中，种植了以下蘑菇：平菇、香菇、裸盖菇、毒伞菌、日本灯霉菌和裸菌。最终，真姬菇和裸盖菇的栽培产生了生长良好的蘑菇。对于古巴裸盖菇来说，裸盖菇素和裸盖菇素是主要的致幻成分。我们观察了储存对这两种致幻剂浓度的影响。从收获古巴裸盖菇开始，冷冻方法为储存蘑菇提供了最有价值的结果。对有毒案例中的样本进行了分析，表明食用了迷幻蘑菇。这种蘑菇被描述为 Psilocybe subcubensis。最近，世界各地对迷幻蘑菇中毒的关注似乎与日俱增。本研究试图推动迷幻蘑菇案件的调查流程。

项目成果

権守邦夫,吉岡尚文: "当教室で経験したきのこ中毒検査事例"秋田県救命救急研究会誌. 5. 74-78 (2001)

Kunio Gomori、Naofumi Yoshioka：“在我们的课堂上经历的蘑菇中毒测试案例”秋田县重症监护研究会杂志5. 74-78（2001）。

KUNIO GONMORI, NAOFUMI YOSHIOKA: "Poisoning Cases by Mushroom"Journal of Akia Emergency Medicine. 5. 74-78 (2001)

KUNIO GONMORI、NAOFUMI YOSHIOKA：“蘑菇中毒病例”Akia 急诊医学杂志。