Development of the Analytical Methods of the Mushroom Toxin

蘑菇毒素分析方法的发展

• 批准号: 63570267
• 负责人: GONMORI Kunio
• 金额: $ 1.34万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570267/
• 关键词: Mushroom toxin; 5-HT; Dopamine; R. rhodopolius; R. crassipes; HPLC; Electro-chemical detector; 高速液体クロマトグラフィ-; セロトニン; モノアミンオキシダーゼ; 電気化学的検出器

Rhodophyllus rhodopolius (Fr.) Quel. has been known in Japan as a poisonous mushroom. According to the annual statistical data from Japanese Ministry of Health and Welfare, R. rhodopolius came in the second to cause poisoning by mushrooms. The Rhodophyllus species are generally harmless, and the toxic R. rhodopolius is often mistaken by amateur mushroom hunters for the edible mushroom Rhodophyllus crassipes, since both species are morphologically very similar. The development of the sensitive and simple methods for routinely discriminating the mushroom between edible and poisonous species are required.In this report the effective methods by means of coloring reagent, spectrophotometry and high-performance liquid chromatography with electro-chemical detector are described. And also the affect of mushroom toxin for the biogenic amines in rat in vitro and in vivo experiments are studied. As the results, the coloring reagent cannot discriminate the mushrooms between edible and poisonous species. On the other hand the use of high-performance liquid chromatography with electro- chemical detector might be practical for distinguishing the R. rhodopolius from R. crassipes. Increased of 5-HT and dopamine content in rat brain were observed by in vivo experiments. But further experiments must be required to find out the mechanism.

Rhodophyllus rhodopolius (Fr.) Quel。在日本被称为有毒蘑菇。据日本厚生省年度统计数据显示，红蕈菌在引起蘑菇中毒的事件中名列第二。红叶菌通常是无害的，而有毒的红叶菌经常被业余蘑菇猎人误认为是可食用的红叶菌，因为这两个物种在形态上非常相似。需要开发灵敏、简单的方法来常规区分蘑菇的食用菌和有毒菌种。本报告介绍了显色剂、分光光度法和电化学检测器高效液相色谱法等有效方法。并通过大鼠体内外实验研究了蘑菇毒素对生物胺的影响。结果，着色试剂无法区分蘑菇的食用菌和有毒菌。另一方面，使用带有电化学检测器的高效液相色谱法对于区分 R. rhodopolius 和 R. crassipes 可能是实用的。体内实验观察到大鼠脑内5-HT和多巴胺含量增加。但必须需要进一步的实验来找出其中的机制。

项目成果

権守邦夫,高橋建吉: "脳内アミンへのキノコ毒の影響について" 第73次日本法医学会総会(口頭発表)岡山市. (1989)

Kunio Gomori、Kenkichi Takahashi：“论蘑菇毒物对大脑中胺的影响”，第 73 届日本法医学会年会（口头报告），冈山市（1989 年）。

Kunio Gonmori, Naofumi Yoshioka: "Effect of Mushroom Toxin on Biogenic Amines of Rat Brain" The Japanese Journal of Legal Medicine, 44, (1989) in press.

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒素对大鼠大脑生物胺的影响”《日本法医学杂志》，44，（1989）正在出版。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 第74次日本法医学会総会(口頭発表)那覇市. (1990)

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒素对生理活性胺的影响”，第 74 届日本法医学会年会（口头报告），那霸市（1990 年）。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 日本法医学雑誌.

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒物对生理活性胺的影响”日本法医学杂志。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 日本法医学雑誌. 44. (1990)

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒物对生理活性胺的影响”《日本法医学杂志》44。（1990）