Development of the Analytical Methods of the Mushroom Toxin

蘑菇毒素分析方法的发展

• 批准号: 63570267
• 负责人: GONMORI Kunio
• 金额: $ 1.34万
• 依托单位: Akita University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1988
• 资助国家: 日本
• 起止时间: 1988 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570267/
• 关键词: Mushroom toxin; 5-HT; Dopamine; R. rhodopolius; R. crassipes; HPLC; Electro-chemical detector; 高速液体クロマトグラフィ-; セロトニン; モノアミンオキシダーゼ; 電気化学的検出器

Rhodophyllus rhodopolius (Fr.) Quel. has been known in Japan as a poisonous mushroom. According to the annual statistical data from Japanese Ministry of Health and Welfare, R. rhodopolius came in the second to cause poisoning by mushrooms. The Rhodophyllus species are generally harmless, and the toxic R. rhodopolius is often mistaken by amateur mushroom hunters for the edible mushroom Rhodophyllus crassipes, since both species are morphologically very similar. The development of the sensitive and simple methods for routinely discriminating the mushroom between edible and poisonous species are required.In this report the effective methods by means of coloring reagent, spectrophotometry and high-performance liquid chromatography with electro-chemical detector are described. And also the affect of mushroom toxin for the biogenic amines in rat in vitro and in vivo experiments are studied. As the results, the coloring reagent cannot discriminate the mushrooms between edible and poisonous species. On the other hand the use of high-performance liquid chromatography with electro- chemical detector might be practical for distinguishing the R. rhodopolius from R. crassipes. Increased of 5-HT and dopamine content in rat brain were observed by in vivo experiments. But further experiments must be required to find out the mechanism.

Rhodophyllus Rhodopolius（Fr.）Quel。在日本被称为有毒蘑菇。根据日本卫生和福利部的年度统计数据，R。R. R. R. R. R. R. popopy第二次引起蘑菇中毒。杜鹃花通常是无害的，有毒的R. R. R. R. R. R. R. R. R. R. R. R. R. R. surgeroom Hunters将食用的蘑菇Rhodophyllus crassipes误认为，因为这两种物种在形态上都非常相似。需要敏感和简单的方法，以常规区分可食用物种和有毒物种之间的蘑菇。在本报告中，描述了通过着色试剂，分光光度法和高性能液相色谱与电化学检测器的有效方法。还研究了蘑菇毒素对大鼠体外和体内实验中生物胺的影响。作为结果，着色试剂不能区分可食用物种和有毒物种之间的蘑菇。另一方面，用电探测器使用高性能液相色谱法可能是将R. r. r. r. ropolius和r。crassipes区分开的实用性。通过体内实验观察到大鼠脑中5-HT和多巴胺含量的增加。但是必须需要进一步的实验才能找出机制。

项目成果

Kunio Gonmori, Naofumi Yoshioka: "Effect of Mushroom Toxin on Biogenic Amines of Rat Brain" The Japanese Journal of Legal Medicine, 44, (1989) in press.

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒素对大鼠大脑生物胺的影响”《日本法医学杂志》，44，（1989）正在出版。

権守邦夫,高橋建吉: "脳内アミンへのキノコ毒の影響について" 第73次日本法医学会総会(口頭発表)岡山市. (1989)

Kunio Gomori、Kenkichi Takahashi：“论蘑菇毒物对大脑中胺的影响”，第 73 届日本法医学会年会（口头报告），冈山市（1989 年）。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 日本法医学雑誌.

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒物对生理活性胺的影响”日本法医学杂志。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 第74次日本法医学会総会(口頭発表)那覇市. (1990)

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒素对生理活性胺的影响”，第 74 届日本法医学会年会（口头报告），那霸市（1990 年）。

権守邦夫,吉岡尚文: "キノコ毒の生理活性アミンへの影響" 日本法医学雑誌. 44. (1990)

Kunio Gomori、Naofumi Yoshioka：“蘑菇毒物对生理活性胺的影响”《日本法医学杂志》44。（1990）