Three-dimensional analysis for neural network of photosensory pineal organ

松果体光感神经网络的三维分析

基本信息

批准号：
10640663
负责人：
TAMOTSU Satoshi
金额：
$ 2.18万
依托单位：
Nara Women's University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

In the pineal organ of river lamprey, Lampetra japonica, there are two types pineal photoreccptors, typical and modified, which are regarded as the pure photoreceptor cell like visual cells in retina and the photoneuroendocrine cell secreting the melatonin, respectively. We investigate for the structure and the subtype of pineal photoreceptors and ganglion cells and their neural network three-dimensionally by use of a fluorescent labeling technique and a confocal laser-scanning microscope. The results are summarized below.1. The photoneuroendocrine cell, which shows serotonin immunoreactivity, possesses a basal process, and it appears that the process does not branch out. These processes contact each other at the basal region of the end vesicle, .2. Most of serotonin-immunoreactive cells showed iodopsin immunoreactivity, but not all. The results suggest that the iodopsin (red-cone visual pigment)-immunoreactive cells are predominant in the end-vesicle of lamprey pineal organ as neuroendocrine cells.3. Some visinin (cone-specific Ca2+binding protein)-immunoreactive cells show no iodopsin immunoreactivity. Many visinin-immunoreactive cells showed rhodopsin immunoreactivity. The results suggest that another cone-type photoreceptor exits in the pineal complex besides iodopsin immunoreactive cells.4. Globular and large ganglion cells were mainly located at peripheral region of the end vesicle. The ganglion cells connecting with the visinin-immunoreactive cells were globular in shape and large in size.

在lampetra japonica的河lamp子的松果体中，有两种类型的松果体光电蛋白酶，典型和修饰，它们被视为视网膜中的纯感光细胞，如视觉细胞，分别是分泌褪黑激素的光尿内分泌细胞。我们通过使用荧光标记技术和共聚焦激光扫描显微镜来研究松果感受器和神经节细胞的结构和亚型及其神经网络。结果总结为1。表现出5-羟色胺免疫反应性的光开肠内内分泌细胞具有基础过程，看来该过程没有分支。这些过程在末端囊泡的基础区域相互接触，.2。大多数5-羟色胺免疫反应性细胞显示出碘的免疫反应性，但不是全部。结果表明，碘播（红锥视觉色素） - 免疫反应性细胞主要是lamp鼠松果体器官作为神经内分泌细胞的末端。3。某些粘蛋白（锥体特异性Ca2+结合蛋白） - 免疫反应性细胞没有碘接种蛋白免疫反应性。许多粘蛋白免疫反应性细胞显示出视紫红质的免疫反应性。结果表明，另一个锥体型光感受器在松果体复合物中退出外，除了碘蛋白酶免疫反应性细胞外。4。球状和大神经节细胞主要位于末端囊泡的外围区域。与粘蛋白免疫反应性细胞连接的神经节细胞的形状是球形的，大小较大。