Bactericidal principle of the fermented aqueous extract of Stevia rebaudiana Bertoni and a possible use of the Stevia extract as a feed supplement for prevention of the digestive tract infections

甜叶菊发酵水提取物的杀菌原理以及甜叶菊提取物作为预防消化道感染的饲料补充剂的可能用途

基本信息

批准号：
10556014
负责人：
KAMIO Yoshiyuki
金额：
$ 8.58万
依托单位：
TOHOKU UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

项目摘要

We have previously shown that a fermenteds extract from the Paraguayan composite plant, Stevia rebaudiana Bertoni has strong bactericidal activity towards a wide range of food-borne pathogenic bacteria, but it shows no significant killing activity to Bifidobacteria and Lactobacilli. Here we studied bactericidal principle of the fermented extract, and tested the extract for possible use as a feed supplement for prevention of the intestinal tract infections of domestic animals.1. Fermented aqueous extract of Stevia rebaudiana Bertoni was fractionated by the partition with ethyl acetate, followed by the high performance column chromatography using an ODS (CィイD218ィエD2) column. Two bactericidal fractions and one enhancer fraction were obtained. Viability of E. coli was reduced from 10ィイD19ィエD1 cfu/ml to <10ィイD13ィエD1 cfu/ml when incubated with the bactericidal fractions at 37℃ for 1h. One bactericidal fraction has he maximal absorption at 280 nm, and it was sensitive to light irradiation. The other bactericidal fraction was identified as lactic acid.2. In the laboratory experiments, oral administration of the Stevia extract to the Listeria-infected mice decreased the numbers of viable bacteria in the intestinal tract from 10ィイD17ィエD1 cfu/ml to 10ィイD13ィエD1 cfu/ml within 3 days. Oral administration of the extract to the Salmonella-infected calves reduced viable bacteria in the intestinal tract and bacterial tissue invasion.3. In the field experiments, oral administration of the Stevia extract was effective to the intestinal tract infections of piggys. Many of the animals with early-staged diarrhea were recovered within 3 days after the treatment, and their growth rate was normal. Oral administration of the diluted Stevia extract promoted growth of pigs, and the quality of the pork meat was evaluated high at the slaughter house.

我们先前表明，来自巴拉圭复合植物的发酵提取物具有广泛的食物传播致病性细菌，但没有显示出对双歧杆菌和乳酸杆菌的杀伤活性。补充了使用n d218 yie D2的肠道肠道感染。 E D1 CFU/ML在37°C下与bacterical ractions孵育1小时。从10 Yi D17 YIE D1 CFU/ML到10 I D13 YIER D1 CFU/ML的可行细菌肠道数量在3天内口服沙门氏菌感染的血液中，可降低肠道和Bactorial Tisue.3田间实验，甜叶菊提取物的口服给予猪的肠道感染。在Slauthter的房子里。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Toshio TOMITA, Naohiko SATO, Minoru SATO, Hiroyuki SHIRAISHI, Masayuki TAKEUCHI, and Yoshiyuki KAMIO: "Bactericidal activity of a fermented extract of Stevia rebaudiana Bertoni towards enterohaemorrhagic Escherichiia colli and other food-borne pathogenic

Toshio TOMITA、Naohiko SATO、Minoru SATO、Hiroyuki Shiraishi、Masayuki TAKEUCHI 和 Yoshiyuki KAMIO：“甜叶菊发酵提取物对肠出血性大肠杆菌和其他食源性致病菌的杀菌活性

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富田敏夫、神尾好是、佐藤直彦: "食中毒菌に対する選択的殺菌剤"公開特許広報特開平11-43443. (1999)

Toshio Tomita、Yoshiko Kamio、Naohiko Sato：“针对食物中毒细菌的选择性消毒剂”专利公开 JP-A-11-43443 (1999)。

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0
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冨田敏夫、佐藤直彦、佐藤*、荒井富雄、白石廣行、神尾好是: "腸管出血性大腸菌O157:H7及び他の食中毒起因菌に対するステビア発酵液の殺菌効果"日本農芸化学会誌(大会講演要旨集). 72. 126 (1998)

Toshio Tomita、Naohiko Sato、Sato*、Tomio Arai、Hiroyuki Shiraishi、Yoshizuru Kamio：“甜叶菊发酵液对肠出血性大肠杆菌 O157:H7 和其他引起食物中毒的细菌的杀菌作用”日本农业化学学会杂志（会议）摘要）) 72. 126 (1998)。