Bactericidal principle of the fermented aqueous extract of Stevia rebaudiana Bertoni and a possible use of the Stevia extract as a feed supplemen for prevention of the digestive tract infections.

甜叶菊发酵水提取物的杀菌原理以及甜叶菊提取物作为预防消化道感染的饲料补充剂的可能用途。

• 批准号: 12556010
• 负责人: KAMIO Yoshiyuki
• 金额: $ 8.51万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-12556010/
• 关键词: Stevia rebaudiana Bertoni; bactercidal substances; fermehted extract; domestic animals; bacterial infection; feed supplement; digestive tract; growth promotion; 細菌感染

We have previously shown that a fermenteds extract from the Paraguayan composite plant, Stevia rebaudiana Bertoni has strong bactericidal activity towards a wide range of food-borne pathogenic bacteria, but it shows no significant killing activity to Bifidobacteria and Lactobacilli. Here we studied bactericidal principle of the fermented extract, and tested the extract for possible use as a feed supplement for prevention of the intestinal tract infections of domestic animals and for promotion of animal growth.1. Fermented aqueous extract of Stevia rebaudiana Bertoni was fractionated by the partition with ethyl acetate, followed by the high performance column chromatography using an ODS (C_<18>) column. Two bactericidal fractions and one enhancer fraction were obtained. Viability of E. coli was reduced from 10^9 cfu/ml to <10^3 cfu/ml when incubated with the bactericidal fractions at 37℃ for 1 h. One bactericidal fraction has the maximal absroption at 280 nm, and it was sensitive to light irradiation. The other bactericidal fraction was identified as lactic acid.2. In the laboratory experiments, oral administration of the Stevia extract to the Salmonella-infected calves reduced viable bacteria in the intestinal tract and bacterial tissue invasion, although not prevented the lethal effect of the bacterium.3. In the field experiments, oral administration of the Stevia extract was effective to the intestinal tract infections of piggys. Many of the animals with early-staged diarrhea were recovered within 3 days after the treatment, and their growth rate was normal threeafter. Oral adminitration of the diluted Stevia extract promoted growth of pigs. Livers of the Stervia-fed pigs were evaluated to be very healthy at the slaughter house. Taste of the pork meat was exellent.

我们先前表明，来自巴拉圭复合植物的发酵提取物具有广泛的食物传播致病性细菌，但没有显示出对双歧杆菌和乳酸杆菌的杀伤活性。补充术的肠道感染和动物生长的促进。1使用ODS（C_ <18>）。在37°C下与细菌分数一起在280 nm HT辐射下，在37°C下与细菌分数孵育1小时实验，肠道和tisue的口服降低了肠道和tisue的活力降低，虽然没有阻止野外经验的致命作用。3。猪的肠道感染是正常的，以下是稀释的甜叶菊提取物的口服。

项目成果

K.Yokota and Y.Kamio: "Tyrosine72 residue at the bottom of rim domain in LukF crucial for the sequential binding of the staphylococcal γ-hemolysin to human erythrocytes"Biosci.Biotechnol.Biochem.. 64(12). 2744-2747 (2000)

K.Yokota 和 Y.Kamio：“LukF 中 rim 结构域底部的酪氨酸 72 残基对于葡萄球菌 γ-溶血素与人红细胞的顺序结合至关重要”Biosci.Biotechnol.Biochem.. 64(12)。 2000)

Nguyen,A.H.,Tomita,T.,Hayashi,T.,and Kamio,Y.: "A DNA inversion in the tail fiber gene alters the host range specificity of carotovoricin Er, a phage-tail-like bacteriocin of Erwinia carotovora subsp.carotovora Er."Mol.Microbiol.. (in press.). (2001)

Nguyen,A.H.、Tomita,T.、Hayashi,T. 和 Kamio,Y.：“尾部纤维基因中的 DNA 倒置改变了胡萝卜素 Er 的宿主范围特异性，胡萝卜素 Er 是一种胡萝卜软腐欧文氏菌亚种的噬菌体尾样细菌素。

Sawada, H., Yanagida, K., Sugiya, M., and Tomita, T.: "Antibacterial activity of novel fluoroalkyl end-capped cooligomers containing dimethyl(octyl)ammonium segments"Eur. Polym. J.. 57. 1433-1440 (2001)

Sawada, H.、Yanagida, K.、Sugiya, M. 和 Tomita, T.：“含有二甲基（辛基）铵链段的新型氟烷基封端低聚物的抗菌活性”Eur。

Y. Yamamoto, L. B. Poole, R. R. Hantgan, Y. Kamio: "An iron-binding protein, Dpr from Streptococcus mutans prevents iron-dependent hydroxyl radical formation in vitro"J. Bacteriol.. (in press). (2002)

Y. Yamamoto、L. B. Poole、R. R. Hantgan、Y. Kamio：“来自变形链球菌的铁结合蛋白 Dpr 可在体外防止铁依赖性羟基自由基的形成”J.

K. Nakamura, T. Tomita, N. Abe, and Y, Kamio: "Purification and characterization of an extracellular poly (L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K 104-1"Appl. Envirn. Microbiol.. 67. 345-353 (2001)

K. Nakamura、T. Tomita、N. Abe 和 Y, Kamio：“来自土壤分离物 Amycolatopsis sp. K 104-1 菌株的细胞外聚（L-乳酸）解聚酶的纯化和表征”Appl。